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Metabonomics-based analysis of Brachyspira pilosicoli's response to tiamulin reveals metabolic activity despite significant growth inhibition

Le Roy, CI, Passey, Jade, Woodward, MJ, La Ragione, Roberto and Claus, SP (2017) Metabonomics-based analysis of Brachyspira pilosicoli's response to tiamulin reveals metabolic activity despite significant growth inhibition Anaerobe, 45. pp. 71-77.

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Abstract

Pathogenic anaerobes Brachyspira spp. are responsible for an increasing number of Intestinal Spirochaetosis (IS) cases in livestock against which few approved treatments are available. Tiamulin is used to treat swine dysentery caused by Brachyspira spp. and recently has been used to handle avian intestinal spirochaetosis (AIS). The therapeutic dose used in chickens requires further evaluation since cases of bacterial resistance to tiamulin have been reported. In this study, we evaluated the impact of tiamulin at varying concentrations on the metabolism of B. pilosicoli using a 1 H-NMR-based metabonomics approach allowing the capture of the overall bacterial metabolic response to antibiotic treatment. Based on growth curve studies, tiamulin impacted bacterial growth even at very low concentration (0.008 mg/mL) although its metabolic activity was barely affected 72 h post exposure to antibiotic treatment. Only the highest dose of tiamulin tested (0.250 mg/mL) caused a major metabolic shift. Results showed that below this concentration, bacteria could maintain a normal metabolic trajectory despite significant growth inhibition by the antibiotic, which may contribute to disease reemergence post antibiotic treatment. Indeed, we confirmed that B. pilosicoli remained viable even after exposition to the highest antibiotic dose. This paper stresses the need to ensure new evaluation of bacterial viability post bacteriostatic exposure such as tiamulin to guarantee treatment efficacy and decrease antibiotic resistance development

Item Type: Article
Subjects : Veterinary Medicine
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
NameEmailORCID
Le Roy, CIUNSPECIFIEDUNSPECIFIED
Passey, Jadej.passey@surrey.ac.ukUNSPECIFIED
Woodward, MJUNSPECIFIEDUNSPECIFIED
La Ragione, RobertoR.Laragione@surrey.ac.ukUNSPECIFIED
Claus, SPUNSPECIFIEDUNSPECIFIED
Date : 31 March 2017
Identification Number : 10.1016/j.anaerobe.2017.03.018
Copyright Disclaimer : © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Uncontrolled Keywords : Metabonomics Brachyspira pilosicoli Tiamulin Antibiotics
Depositing User : Symplectic Elements
Date Deposited : 07 Apr 2017 11:34
Last Modified : 11 Jul 2017 10:54
URI: http://epubs.surrey.ac.uk/id/eprint/813985

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