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Refined analysis of the Campylobacter jejuni iron-dependent/independent Fur- and PerR-transcriptomes

Butcher, J, Handley, RA, van Vliet, AH and Stintzi, A (2015) Refined analysis of the Campylobacter jejuni iron-dependent/independent Fur- and PerR-transcriptomes BMC Genomics, 16, 498.

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Abstract

Background The genome of Campylobacter jejuni contains two iron activated Fur-family transcriptional regulators, CjFur and CjPerR, which are primarily responsible for regulating iron homeostasis and oxidative stress respectively. Both transcriptional regulators have been previously implicated in regulating diverse functions beyond their primary roles in C. jejuni. To further characterize their regulatory networks, RNA-seq was used to define the transcriptional profiles of C. jejuni NCTC11168 wild type, Δfur, ΔperR and ΔfurΔperR isogenic deletion mutants under both iron-replete and iron-limited conditions. Results It was found that 202 genes were differentially expressed in at least one mutant under iron-replete conditions and 331 genes were differentially expressed in at least one mutant under iron-limited conditions. The CjFur and CjPerR transcriptomes characterized in this study were compared to those previously identified using microarray profiling and found to be more extensive than previously understood. Interestingly, our results indicate that CjFur/CjPerR appear to co-regulate the expression of flagellar biogenesis genes in an opposing and iron-independent fashion. Moreover the ΔfurΔperR isogenic deletion mutant revealed that CjFur and CjPerR can compensate for each other in certain cases, suggesting that both regulators may compete for binding to specific promoters. Conclusions The CjFur and CjPerR transcriptomes are larger than previously reported. In particular, deletion of perR results in the differential expression of a large group of genes in the absence of iron, suggesting that CjPerR may also regulate genes in an iron-independent manner, similar to what has already been demonstrated with CjFur. Moreover, subsets of genes were found which are only differentially expressed when both CjFur and CjPerR are deleted and includes genes that appear to be simultaneously activated by CjFur and repressed by CjPerR. In particular the iron-independent co-regulation of flagellar biogenesis by CjFur/CjPerR represents a potentially novel regulatory function for these proteins. These findings represent additional modes of co-regulation by these two transcriptional regulators in C. jejuni.

Item Type: Article
Subjects : Veterinary Medicine
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
AuthorsEmailORCID
Butcher, JUNSPECIFIEDUNSPECIFIED
Handley, RAUNSPECIFIEDUNSPECIFIED
van Vliet, AHUNSPECIFIEDUNSPECIFIED
Stintzi, AUNSPECIFIEDUNSPECIFIED
Date : 4 July 2015
Identification Number : https://doi.org/10.1186/s12864-015-1661-7
Copyright Disclaimer : © Butcher et al. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated
Uncontrolled Keywords : Fur PerR Co-regulation Iron-independent regulation
Depositing User : Symplectic Elements
Date Deposited : 04 Apr 2017 10:55
Last Modified : 04 Apr 2017 10:55
URI: http://epubs.surrey.ac.uk/id/eprint/813925

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