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Conservation of sigma28-Dependent Non-Coding RNA Paralogs and Predicted sigma54-Dependent Targets in Thermophilic Campylobacter Species

Le, MT, van Veldhuizen, M, Porcelli, I, Bongaerts, RJ, Gaskin, DJ, Pearson, BM and van Vliet, AH (2015) Conservation of sigma28-Dependent Non-Coding RNA Paralogs and Predicted sigma54-Dependent Targets in Thermophilic Campylobacter Species PLoS One, 10 (10).

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Abstract

Assembly of flagella requires strict hierarchical and temporal control via flagellar sigma and anti-sigma factors, regulatory proteins and the assembly complex itself, but to date non-coding RNAs (ncRNAs) have not been described to regulate genes directly involved in flagellar assembly. In this study we have investigated the possible role of two ncRNA paralogs (CjNC1, CjNC4) in flagellar assembly and gene regulation of the diarrhoeal pathogen Campylobacter jejuni. CjNC1 and CjNC4 are 37/44 nt identical and predicted to target the 5' untranslated region (5' UTR) of genes transcribed from the flagellar sigma factor σ54. Orthologs of the σ54-dependent 5' UTRs and ncRNAs are present in the genomes of other thermophilic Campylobacter species, and transcription of CjNC1 and CNC4 is dependent on the flagellar sigma factor σ28. Surprisingly, inactivation and overexpression of CjNC1 and CjNC4 did not affect growth, motility or flagella-associated phenotypes such as autoagglutination. However, CjNC1 and CjNC4 were able to mediate sequence-dependent, but Hfq-independent, partial repression of fluorescence of predicted target 5' UTRs in an Escherichia coli-based GFP reporter gene system. This hints towards a subtle role for the CjNC1 and CjNC4 ncRNAs in post-transcriptional gene regulation in thermophilic Campylobacter species, and suggests that the currently used phenotypic methodologies are insufficiently sensitive to detect such subtle phenotypes. The lack of a role of Hfq in the E. coli GFP-based system indicates that the CjNC1 and CjNC4 ncRNAs may mediate post-transcriptional gene regulation in ways that do not conform to the paradigms obtained from the Enterobacteriaceae.

Item Type: Article
Subjects : Veterinary Medicine
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
AuthorsEmailORCID
Le, MTUNSPECIFIEDUNSPECIFIED
van Veldhuizen, MUNSPECIFIEDUNSPECIFIED
Porcelli, IUNSPECIFIEDUNSPECIFIED
Bongaerts, RJUNSPECIFIEDUNSPECIFIED
Gaskin, DJUNSPECIFIEDUNSPECIFIED
Pearson, BMUNSPECIFIEDUNSPECIFIED
van Vliet, AHUNSPECIFIEDUNSPECIFIED
Date : 29 October 2015
Identification Number : https://doi.org/10.1371/journal.pone.0141627
Copyright Disclaimer : © 2015 Le et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Depositing User : Symplectic Elements
Date Deposited : 04 Apr 2017 10:33
Last Modified : 04 Apr 2017 10:33
URI: http://epubs.surrey.ac.uk/id/eprint/813922

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