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Reflection imaging of China ink-perfused brain vasculature using confocal laser-scanning microscopy after clarification of brain tissue by the Spalteholz method

Gutierre, RC, Vannucci Campos, D, Mortara, RA, Coppi, Augusto and Arida, RM (2017) Reflection imaging of China ink-perfused brain vasculature using confocal laser-scanning microscopy after clarification of brain tissue by the Spalteholz method Journal of Anatomy, 230 (4). pp. 601-606.

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Abstract

Confocal laser-scanning microscopy is a useful tool for visualizing neurons and glia in transparent preparations of brain tissue from laboratory animals. Currently, imaging capillaries and venules in transparent brain tissues requires the use of fluorescent proteins. Here, we show that vessels can be imaged by confocal laser-scanning microscopy in transparent cortical, hippocampal and cerebellar preparations after clarification of China ink-injected specimens by the Spalteholz method. This method may be suitable for global, three-dimensional, quantitative analyses of vessels, including stereological estimations of total volume and length and of surface area of vessels, which constitute indirect approaches to investigate angiogenesis.

Item Type: Article
Subjects : Veterinary Medicine
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
NameEmailORCID
Gutierre, RCUNSPECIFIEDUNSPECIFIED
Vannucci Campos, DUNSPECIFIEDUNSPECIFIED
Mortara, RAUNSPECIFIEDUNSPECIFIED
Coppi, Augustoa.coppi@surrey.ac.ukUNSPECIFIED
Arida, RMUNSPECIFIEDUNSPECIFIED
Date : 10 March 2017
Identification Number : 10.1111/joa.12578
Copyright Disclaimer : This is the peer reviewed version of the following article: Gutierre, R. C., Vannucci Campos, D., Mortara, R. A., Coppi, A. A. and Arida, R. M. (2017), Reflection imaging of China ink-perfused brain vasculature using confocal laser-scanning microscopy after clarification of brain tissue by the Spalteholz method. J. Anat., 230: 601–606. doi:10.1111/joa.12578, which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1111/joa.12578/abstract. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Depositing User : Symplectic Elements
Date Deposited : 28 Mar 2017 13:49
Last Modified : 19 Jul 2017 13:52
URI: http://epubs.surrey.ac.uk/id/eprint/813867

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