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Electrospun oriented gelatin-hydroxyapatite fibre scaffolds for bone tissue engineering

Salifu, AA, Lekakou, Constantina and Labeed, Fatima (2017) Electrospun oriented gelatin-hydroxyapatite fibre scaffolds for bone tissue engineering Journal of Biomedical Materials Research Part A, 105 (7). pp. 1911-1926.

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Abstract

Tissue engineering of human foetal osteoblast (hFOB) cells was investigated on gelatin-hydroxyapatite (HA), crosslinked, electrospun oriented fibre scaffolds at the different hydroxyapatite concentrations of 0, 10, 20 and 25 wt% in the dry fibres and different fibre diameter, pore size and porosity of scaffolds. Rheological tests and proton NMR spectroscopy were conducted for all solutions used for electrospinning. It was found that 25 wt% HA-gelatin scaffolds electrospun at 20 kV led to the greatest cell attachment, cell proliferation and extracellular matrix (ECM) production while fibre orientation improved the mechanical properties, where crosslinked electrospun 25 wt% HA-gelatin fibre scaffolds yielded a Young’s modulus in the range of 0.5 to 0.9 GPa and a tensile strength in the range of 4 to 10 MPa in the fibre direction for an applied voltage of 20 to 30 kV, respectively, in the electrospinning of scaffolds. Biological characterisation of cell seeded scaffolds yielded the rate of cell growth and ECM (collagen and calcium) production by the cells as a function of time; it included cell seeding efficiency tests, alamar blue cell proliferation assay, alkaline phosphate (ALP) assay, collagen assay, calcium colorimetric assay, fluorescence microscopy for live and dead cells, and scanning electron microscopy (SEM) for cell culture from 1 to 18 days. After 18 days, cells seeded and grown on the 25 wt% HA-gelatin scaffold, electrospun at 20 kV, reached production of collagen at 370 g/L and calcium production at 0.8 mM.

Item Type: Article
Subjects : Mechanical Engineering Science
Divisions : Faculty of Engineering and Physical Sciences > Mechanical Engineering Sciences
Authors :
NameEmailORCID
Salifu, AAUNSPECIFIEDUNSPECIFIED
Lekakou, ConstantinaC.Lekakou@surrey.ac.ukUNSPECIFIED
Labeed, FatimaF.Labeed@surrey.ac.ukUNSPECIFIED
Date : 7 June 2017
Identification Number : 10.1002/jbm.a.36058
Copyright Disclaimer : This is the peer reviewed version of the following article: Salifu AA, Lekakou C, Labeed FH. 2017. Electrospun oriented gelatin-hydroxyapatite fiber scaffolds for bone tissue engineering . J Biomed Mater Res Part A 2017:105A:1911–1926, which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1002/jbm.a.36058/full. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Uncontrolled Keywords : tissue engineering; osteoblasts; gelatin-hydroxyapatite; electrospinning; scaffolds; mechanical testing; biological characterisation.
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 07 Mar 2017 08:42
Last Modified : 05 Jul 2017 11:35
URI: http://epubs.surrey.ac.uk/id/eprint/813697

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