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Phenotypic microarrays suggest Escherichia coli ST131 is not a metabolically distinct lineage of extra-intestinal pathogenic E. coli.

Alqasim, A, Emes, R, Clark, G, Newcombe, J, La Ragione, R and McNally, A (2014) Phenotypic microarrays suggest Escherichia coli ST131 is not a metabolically distinct lineage of extra-intestinal pathogenic E. coli. PloS one, 9 (2). e88374-e88374.

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Abstract

Extraintestinal pathogenic E. coli (ExPEC) are the major aetiological agent of urinary tract infections (UTIs) in humans. The emergence of the CTX-M producing clone E. coli ST131 represents a major challenge to public health worldwide. A recent study on the metabolic potential of E. coli isolates demonstrated an association between the E. coli ST131 clone and enhanced utilisation of a panel of metabolic substrates. The studies presented here investigated the metabolic potential of ST131 and other major ExPEC ST isolates using 120 API test reagents and found that ST131 isolates demonstrated a lower metabolic activity for 5 of 120 biochemical tests in comparison to non-ST131 ExPEC isolates. Furthermore, comparative phenotypic microarray analysis showed a lack of specific metabolic profile for ST131 isolates countering the suggestion that these bacteria are metabolically fitter and therefore more successful human pathogens.

Item Type: Article
Subjects : Electronic Engineering
Divisions : Faculty of Engineering and Physical Sciences > Electronic Engineering
Authors :
AuthorsEmailORCID
Alqasim, AUNSPECIFIEDUNSPECIFIED
Emes, RUNSPECIFIEDUNSPECIFIED
Clark, GUNSPECIFIEDUNSPECIFIED
Newcombe, JUNSPECIFIEDUNSPECIFIED
La Ragione, RUNSPECIFIEDUNSPECIFIED
McNally, AUNSPECIFIEDUNSPECIFIED
Date : 7 February 2014
Identification Number : https://doi.org/10.1371/journal.pone.0088374
Copyright Disclaimer : © 2014 Alqasim et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Uncontrolled Keywords : Humans, Escherichia coli, Escherichia coli Infections, Urinary Tract Infections, Microarray Analysis, Microbial Sensitivity Tests, Phenotype
Depositing User : Symplectic Elements
Date Deposited : 28 Feb 2017 11:30
Last Modified : 28 Feb 2017 11:30
URI: http://epubs.surrey.ac.uk/id/eprint/813640

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