University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Differences in carbon source utilisation distinguish Campylobacter jejuni from Campylobacter coli.

Wagley, S, Newcombe, J, Laing, E, Yusuf, E, Sambles, CM, Studholme, DJ, La Ragione, RM, Titball, RW and Champion, OL (2014) Differences in carbon source utilisation distinguish Campylobacter jejuni from Campylobacter coli. BMC Microbiology, 14.

[img]
Preview
Text
Differences in carbon source utilisation distinguish Campylobacter jejuni from Campylobacter coli.pdf - Version of Record
Available under License Creative Commons Attribution.

Download (783kB) | Preview
[img]
Preview
Text (licence)
SRI_deposit_agreement.pdf
Available under License : See the attached licence file.

Download (33kB) | Preview

Abstract

BACKGROUND: Campylobacter jejuni and C. coli are human intestinal pathogens that are the most frequent causes of bacterial foodborne gastroenteritis in humans in the UK. In this study, we aimed to characterise the metabolic diversity of both C. jejuni and C. coli using a diverse panel of clinical strains isolated from the UK, Pakistan and Thailand, thereby representing both the developed and developing world. Our aim was to apply multi genome analysis and Biolog phenotyping to determine differences in carbon source utilisation by C. jejuni and C. coli strains. RESULTS: We have identified a core set of carbon sources (utilised by all strains tested) and a set that are differentially utilised for a diverse panel of thirteen C. jejuni and two C. coli strains. This study used multi genome analysis to show that propionic acid is utilised only by C. coli strains tested. A broader PCR screen of 16 C. coli strains and 42 C. jejuni confirmed the absence of the genes needed for propanoate metabolism. CONCLUSIONS: From our analysis we have identified a phenotypic method and two genotypic methods based on propionic utilisation that might be applicable for distinguishing between C. jejuni and C. coli.

Item Type: Article
Subjects : Electronic Engineering
Divisions : Faculty of Engineering and Physical Sciences > Electronic Engineering
Authors :
AuthorsEmailORCID
Wagley, SUNSPECIFIEDUNSPECIFIED
Newcombe, JUNSPECIFIEDUNSPECIFIED
Laing, EUNSPECIFIEDUNSPECIFIED
Yusuf, EUNSPECIFIEDUNSPECIFIED
Sambles, CMUNSPECIFIEDUNSPECIFIED
Studholme, DJUNSPECIFIEDUNSPECIFIED
La Ragione, RMUNSPECIFIEDUNSPECIFIED
Titball, RWUNSPECIFIEDUNSPECIFIED
Champion, OLUNSPECIFIEDUNSPECIFIED
Date : 28 October 2014
Funders : UK Biotechnology and Biological Science Research Council (BBSRC)
Identification Number : https://doi.org/10.1186/s12866-014-0262-y
Copyright Disclaimer : © 2014 Wagley et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Uncontrolled Keywords : Bacterial Typing Techniques, Campylobacter Infections, Campylobacter coli, Campylobacter jejuni, Carbon, DNA, Bacterial, Great Britain, Humans, Metabolic Networks and Pathways, Molecular Diagnostic Techniques, Molecular Sequence Data, Pakistan, Polymerase Chain Reaction, Propionates, Sequence Analysis, DNA, Thailand
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 28 Feb 2017 11:03
Last Modified : 28 Feb 2017 11:03
URI: http://epubs.surrey.ac.uk/id/eprint/813639

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800