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Colonisation of poultry by Salmonella Enteritidis S1400 is reduced by combined administration of Lactobacillus salivarius 59 and Enterococcus faecium PXN-33

Carter, A, Adams, M, La Ragione, Roberto and Woodward, MJ (2016) Colonisation of poultry by Salmonella Enteritidis S1400 is reduced by combined administration of Lactobacillus salivarius 59 and Enterococcus faecium PXN-33 Veterinary Microbiology, 199. pp. 100-107.

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Abstract

Salmonella Enteritidis remains a significant issue within the poultry industry and one potential solution is to use probiotic bacteria to prevent Salmonella colonisation through competitive exclusion (CE). We demonstrate that combined administration of Lactobacillus salivarius 59 and Enterococcus faecium PXN33 were effective competitive excluders of Salmonella Enteritidis S1400 in poultry. Two models were developed to evaluate the efficacy of probiotic where birds received Salmonella Enteritidis S1400 by a) oral gavage and b) sentinel bird to bird transmission. A statistically significant (p < 0.001) 2 log reduction of Salmonella Enteritidis S1400 colonisation was observed in the ileum, caecum and colon at day 43 using combined administration of the two probiotic bacteria. However, no Salmonella Enteritidis S1400 colonisation reduction was observed when either probiotic was administered individually. In the sentinel bird model the combined probiotic administered at days 12 and 20 was more effective than one-off or double administrations at age 1 and 12 days. In vitro cell free culture supernatant studies suggest the mechanism of Salmonella Enteritidis S1400 inhibition was due to a reduction in pH by the probiotic bacteria. Our current study provides further evidence that probiotics can significantly reduce pathogenic bacterial colonisation in poultry and that mixed preparation of probiotics provide superior performance when compared to individual bacterial preparations.

Item Type: Article
Subjects : Veterinary Medicine
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
NameEmailORCID
Carter, AUNSPECIFIEDUNSPECIFIED
Adams, MUNSPECIFIEDUNSPECIFIED
La Ragione, RobertoR.Laragione@surrey.ac.ukUNSPECIFIED
Woodward, MJUNSPECIFIEDUNSPECIFIED
Date : 26 December 2016
Identification Number : 10.1016/j.vetmic.2016.12.029
Copyright Disclaimer : © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Uncontrolled Keywords : Probiotic; Salmonella Enteritidis; Lactobacillus salivarius; Enterococcus faecium; Poultry; Competitive exclusion
Depositing User : Symplectic Elements
Date Deposited : 15 Feb 2017 18:10
Last Modified : 31 Oct 2017 19:08
URI: http://epubs.surrey.ac.uk/id/eprint/813561

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