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Evaluation of hydrophobic chitosan-based particulate formulations of porcine reproductive and respiratory syndrome virus vaccine candidate T cell antigens

Mokhtar, H, Biffar, L, Somavarapu, S, Frossard, J-P, Mcgowan, Sarah, Pedrera, M, Strong, R, Edwards, JC, Garcia-Durán, M, Rodriguez, MJ , Stewart, Graham, Steinbach, Falko and Graham, Simon (2017) Evaluation of hydrophobic chitosan-based particulate formulations of porcine reproductive and respiratory syndrome virus vaccine candidate T cell antigens Veterinary Microbiology.

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Abstract

PRRS control is hampered by the inadequacies of existing vaccines to combat the extreme diversity of circulating viruses. Since immune clearance of PRRSV infection may not be dependent on the development of neutralising antibodies and the identification of broadly- neutralising antibody epitopes have proven elusive we hypothesised that conserved T cell antigens represent potential candidates for development of a novel PRRS vaccine. Previously we had identified the M and NSP5 proteins as well-conserved targets of polyfunctional CD8 and CD4 T cells. To assess their vaccine potential, peptides representing M and NSP5 were encapsulated in hydrophobically-modified chitosan particles adjuvanted by incorporation of a synthetic multi-TLR2/TLR7 agonist and coated with a model B cell PRRSV antigen. For comparison, empty particles and adjuvanted particles encapsulating inactivated PRRSV-1

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
NameEmailORCID
Mokhtar, HUNSPECIFIEDUNSPECIFIED
Biffar, LUNSPECIFIEDUNSPECIFIED
Somavarapu, SUNSPECIFIEDUNSPECIFIED
Frossard, J-PUNSPECIFIEDUNSPECIFIED
Mcgowan, Sarahs.mcgowan@surrey.ac.ukUNSPECIFIED
Pedrera, MUNSPECIFIEDUNSPECIFIED
Strong, RUNSPECIFIEDUNSPECIFIED
Edwards, JCUNSPECIFIEDUNSPECIFIED
Garcia-Durán, MUNSPECIFIEDUNSPECIFIED
Rodriguez, MJUNSPECIFIEDUNSPECIFIED
Stewart, GrahamG.Stewart@surrey.ac.ukUNSPECIFIED
Steinbach, Falkof.steinbach@surrey.ac.ukUNSPECIFIED
Graham, Simons.graham@surrey.ac.ukUNSPECIFIED
Date : 3 February 2017
Identification Number : 10.1016/j.vetmic.2017.01.037
Copyright Disclaimer : © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Uncontrolled Keywords : porcine reproductive and respiratory syndrome virus, chitosan particles, vaccine formulation, T cell,
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 01 Feb 2017 15:30
Last Modified : 19 Jul 2017 09:38
URI: http://epubs.surrey.ac.uk/id/eprint/813429

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