Evaluation of hydrophobic chitosan-based particulate formulations of porcine reproductive and respiratory syndrome virus vaccine candidate T cell antigens
Mokhtar, H, Biffar, L, Somavarapu, S, Frossard, J-P, Mcgowan, Sarah, Pedrera, M, Strong, R, Edwards, JC, Garcia-Durán, M, Rodriguez, MJ , Stewart, Graham, Steinbach, Falko and Graham, Simon (2017) Evaluation of hydrophobic chitosan-based particulate formulations of porcine reproductive and respiratory syndrome virus vaccine candidate T cell antigens Veterinary Microbiology, 209. pp. 66-74.
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Abstract
PRRS control is hampered by the inadequacies of existing vaccines to combat the extreme diversity of circulating viruses. Since immune clearance of PRRSV infection may not be dependent on the development of neutralising antibodies and the identification of broadly- neutralising antibody epitopes have proven elusive we hypothesised that conserved T cell antigens represent potential candidates for development of a novel PRRS vaccine. Previously we had identified the M and NSP5 proteins as well-conserved targets of polyfunctional CD8 and CD4 T cells. To assess their vaccine potential, peptides representing M and NSP5 were encapsulated in hydrophobically-modified chitosan particles adjuvanted by incorporation of a synthetic multi-TLR2/TLR7 agonist and coated with a model B cell PRRSV antigen. For comparison, empty particles and adjuvanted particles encapsulating inactivated PRRSV-1
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Divisions : | Faculty of Health and Medical Sciences > School of Veterinary Medicine | ||||||||||||||||||||||||||||||||||||||||||
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Date : | 3 February 2017 | ||||||||||||||||||||||||||||||||||||||||||
DOI : | 10.1016/j.vetmic.2017.01.037 | ||||||||||||||||||||||||||||||||||||||||||
Copyright Disclaimer : | © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | ||||||||||||||||||||||||||||||||||||||||||
Uncontrolled Keywords : | Porcine reproductive and respiratory syndrome virus; Chitosan particles; Vaccine formulation; T cell | ||||||||||||||||||||||||||||||||||||||||||
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Depositing User : | Symplectic Elements | ||||||||||||||||||||||||||||||||||||||||||
Date Deposited : | 01 Feb 2017 15:30 | ||||||||||||||||||||||||||||||||||||||||||
Last Modified : | 16 Jan 2019 17:11 | ||||||||||||||||||||||||||||||||||||||||||
URI: | http://epubs.surrey.ac.uk/id/eprint/813429 |
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