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Testing of a palatable bait and compatible vaccine carrier for the oral vaccination of European badgers (Meles meles) against tuberculosis

Gowtage, S, Williams, GA, Henderson, R, Aylett, P, MacMorran, D, Palmer, S, Robertson, A, Lesellier, S, Carter, SP and Chambers, Mark (2017) Testing of a palatable bait and compatible vaccine carrier for the oral vaccination of European badgers (Meles meles) against tuberculosis Vaccine, 35 (6). pp. 987-992.

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Abstract

The oral vaccination of wild badgers (Meles meles) with live Bacillus Calmette–Guérin (BCG) is one of the tools being considered for the control of bovine tuberculosis (caused by Mycobacterium bovis) in the UK. The design of a product for oral vaccination requires that numerous, and often competing, conditions are met. These include the need for a highly palatable, but physically stable bait that will meet regulatory requirements, and one which is also compatible with the vaccine formulation; in this case live BCG. In collaboration with two commercial bait companies we have developed a highly attractive and palatable bait recipe designed specifically for European badgers (Meles meles) that meets these requirements. The palatability of different batches of bait was evaluated against a standardised palatable control bait using captive badgers. The physical properties of the bait are described e.g. firmness and colour. The microbial load in the bait was assessed against European and US Pharmacopoeias. The bait was combined with an edible vaccine carrier made of hydrogenated peanut oil in which BCG vaccine was stable during bait manufacture and cold storage, demonstrating <0.5 log10 reduction in titre after 117 weeks’ storage at −20 °C. BCG stability in bait was also evaluated at +4 °C and under simulated environmental conditions (20 °C, 98% Relative Humidity; RH). Finally, iophenoxic acid biomarkers were utilised as a surrogate for the BCG vaccine, to test variants of the vaccine-bait design for their ability to deliver biomarker to the gastrointestinal tract of individual animals. These data provide the first detailed description of a bait-vaccine delivery system developed specifically for the oral vaccination of badgers against Mycobacterium bovis using live BCG.

Item Type: Article
Subjects : Veterinary Medicine
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
NameEmailORCID
Gowtage, SUNSPECIFIEDUNSPECIFIED
Williams, GAUNSPECIFIEDUNSPECIFIED
Henderson, RUNSPECIFIEDUNSPECIFIED
Aylett, PUNSPECIFIEDUNSPECIFIED
MacMorran, DUNSPECIFIEDUNSPECIFIED
Palmer, SUNSPECIFIEDUNSPECIFIED
Robertson, AUNSPECIFIEDUNSPECIFIED
Lesellier, SUNSPECIFIEDUNSPECIFIED
Carter, SPUNSPECIFIEDUNSPECIFIED
Chambers, Markm.chambers@surrey.ac.ukUNSPECIFIED
Date : 7 February 2017
Identification Number : 10.1016/j.vaccine.2016.12.004
Copyright Disclaimer : © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Uncontrolled Keywords : Badger; BCG; Oral vaccination; Bait; Tuberculosis
Depositing User : Symplectic Elements
Date Deposited : 25 Jan 2017 13:05
Last Modified : 31 Oct 2017 19:04
URI: http://epubs.surrey.ac.uk/id/eprint/813361

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