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An ongoing role for Wnt signaling in differentiating melanocytes in vivo

Vibert, L, Aquino, Gerardo, Gehring, I, Subkhankulova, T, Schilling, TF, Rocco, Andrea and Kelsh, RN (2016) An ongoing role for Wnt signaling in differentiating melanocytes in vivo Pigment Cell & Melanoma Research, 30 (2). pp. 219-232.

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Abstract

A role for Wnt signaling in melanocyte specification from neural crest is conserved across vertebrates, but possible ongoing roles in melanocyte differentiation have received little attention. Using a systems biology approach to investigate the gene regulatory network underlying stable melanocyte differentiation in zebrafish highlighted a requirement for a positive feedback loop involving the melanocyte master regulator Mitfa. Here we test the hypothesis that Wnt signaling contributes to that positive feedback. We show firstly that Wnt signaling remains active in differentiating melanocytes and secondly that enhanced Wnt signaling drives elevated transcription of mitfa. We show that chemical activation of the Wnt signaling pathway at early stages of melanocyte development enhances melanocyte specification as expected, but importantly that at later (differentiation) stages it results in altered melanocyte morphology, although melanisation is not obviously affected. Downregulation of Wnt signaling also results in altered melanocyte morphology and organisation. We conclude that Wnt signaling plays a role in regulating ongoing aspects of melanocyte differentiation in zebrafish.

Item Type: Article
Subjects : Biosciences and Medicine
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
NameEmailORCID
Vibert, LUNSPECIFIEDUNSPECIFIED
Aquino, Gerardog.aquino@surrey.ac.ukUNSPECIFIED
Gehring, IUNSPECIFIEDUNSPECIFIED
Subkhankulova, TUNSPECIFIEDUNSPECIFIED
Schilling, TFUNSPECIFIEDUNSPECIFIED
Rocco, AndreaA.Rocco@surrey.ac.ukUNSPECIFIED
Kelsh, RNUNSPECIFIEDUNSPECIFIED
Date : 15 December 2016
Identification Number : 10.1111/pcmr.12568
Copyright Disclaimer : Copyright 2016 The Authors. Pigment Cell & Melonoma Research Published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Uncontrolled Keywords : Wnt signaling/ melanocyte/ specification/ differentiation/ zebrafish/ neural crest/ mitfa
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 25 Jan 2017 09:19
Last Modified : 19 Jul 2017 08:27
URI: http://epubs.surrey.ac.uk/id/eprint/813330

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