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IQGAP1 in Podosome /Invadosome is Involved in the Progression of Glioblastoma Multiforme Depending on the Tumor Status

Rotoli, D, Pérez-Rodríguez, ND, Morales, M, Maeso, M, Ávila, J, Mobasheri, Ali and Martín-Vasallo, P (2017) IQGAP1 in Podosome /Invadosome is Involved in the Progression of Glioblastoma Multiforme Depending on the Tumor Status International Journal of Molecular Sciences, 18 (1). p. 150.

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Abstract

Glioblastoma multiforme (GBM) is the most frequent and aggressive primary brain tumor. GBM is formed by a very heterogeneous astrocyte population, neurons, neovascularization and infiltrating myeloid cells (microglia and monocyte derived macrophages). The IQGAP1 scaffold protein interacts with components of the cytoskeleton, cell adhesion molecules, and several signaling molecules to regulate cell morphology and motility, cell cycle and other cellular functions. IQGAP1 overexpression and delocalization has been observed in several tumors, suggesting a role for this protein in cell proliferation, transformation and invasion. IQGAP1 has been identified as a marker of amplifying cancer cells in GBMs. To determine the involvement of IQGAP1 in the onco-biology of GBM, we performed immunohistochemical confocal microscopic analysis of the IQGAP1 protein in human GBM tissue samples using cell type-specific markers. IQGAP1 immunostaining and subcellular localization was heterogeneous; the protein was located in the plasma membrane and, at variable levels, in nucleus and/or cytosol. Moreover, IQGAP1 positive staining was found in podosome/invadopodia-like structures. IQGAP1+ staining was observed in neurons (Map2+ cells), in cancer stem cells (CSC; nestin+ ) and in several macrophages (CD31+ or Iba1+ ). Our results indicate that the IQGAP1 protein is involved in normal cell physiology as well as oncologic processes.

Item Type: Article
Subjects : Veterinary Medicine
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
NameEmailORCID
Rotoli, DUNSPECIFIEDUNSPECIFIED
Pérez-Rodríguez, NDUNSPECIFIEDUNSPECIFIED
Morales, MUNSPECIFIEDUNSPECIFIED
Maeso, MUNSPECIFIEDUNSPECIFIED
Ávila, JUNSPECIFIEDUNSPECIFIED
Mobasheri, Alia.mobasheri@surrey.ac.ukUNSPECIFIED
Martín-Vasallo, PUNSPECIFIEDUNSPECIFIED
Date : 13 January 2017
Identification Number : 10.3390/ijms18010150
Copyright Disclaimer : © 2017 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
Uncontrolled Keywords : IQGAP1; glioblastoma multiforme (GBM); scaffold protein; podosome/invadosome
Depositing User : Symplectic Elements
Date Deposited : 18 Jan 2017 12:32
Last Modified : 19 Jul 2017 08:26
URI: http://epubs.surrey.ac.uk/id/eprint/813325

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