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A dielectrophoresis-impedance method for protein detection and analysis

Mohamad, AS, Hamzah, R, Hoettges, Kai and Hughes, Michael (2017) A dielectrophoresis-impedance method for protein detection and analysis AIP Advances, 7 (1), 015202.

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Dielectrophoresis (DEP) has increasingly been used for the assessment of the electrical properties of molecular scale objects including proteins, DNA, nanotubes and nanowires. However, whilst techniques have been developed for the electrical characterisation of frequency-dependent DEP response, biomolecular study is usually limited to observation using fluorescent markers, limiting its applicability as a characterisation tool. In this paper we present a label-free, impedance-based method of characterisation applied to the determination of the electrical properties of colloidal protein molecules, specifically Bovine Serum Albumin (BSA). By monitoring the impedance between electrodes as proteins collect, it is shown to be possible to observe multi-dispersion behaviour. A DEP dispersion exhibited at 400 kHz is attributable to the orientational dispersion of the molecule, whilst a second, higher-frequency dispersion is attributed to a Maxwell-Wagner type dispersion; changes in behaviour with medium conductivity suggest that this is strongly influenced by the electrical double layer surrounding the molecule.

Item Type: Article
Subjects : Mechanical Engineering Science
Divisions : Faculty of Engineering and Physical Sciences > Mechanical Engineering Sciences
Authors :
Date : 13 January 2017
Identification Number : 10.1063/1.4974290
Copyright Disclaimer : Copyright 2017 The Authors. All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (
Depositing User : Symplectic Elements
Date Deposited : 18 Jan 2017 11:40
Last Modified : 31 Oct 2017 19:03

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