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The effect of oral vaccination with Mycobacterium bovis BCG on the development of tuberculosis in captive European badgers (Meles meles)

Chambers, MA, Aldwell, F, Williams, GA, Palmer, S, Gowtage, S, Ashford, R, Dalley, D, Davé, D, Weyer, U, Salguero Bodes, Francisco , Nunez, A, Nadian, A, Crawshaw, T, Corner, LAL and Lesellier, S (2017) The effect of oral vaccination with Mycobacterium bovis BCG on the development of tuberculosis in captive European badgers (Meles meles) Frontiers in cellular and infection microbiology, 7 (6).

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Abstract

The European badger (Meles meles) is a reservoir host of Mycobacterium bovis and responsible for a proportion of the tuberculosis (TB) cases seen in cattle in the United Kingdom and Republic of Ireland. An injectable preparation of the bacillus Calmette-Guérin (BCG) vaccine is licensed for use in badgers in the UK and its use forms part of the bovine TB eradication plans of England and Wales. However, there are practical limitations to the widespread application of an injectable vaccine for badgers and a research priority is the development of an oral vaccine deliverable to badgers in bait. Previous studies reported the successful vaccination of badgers with oral preparations of 108 colony forming units (CFU) of both Pasteur and Danish strains of BCG contained within a lipid matrix composed of triglycerides of fatty acids. Protection against TB in these studies was expressed as a reduction in the number and apparent progression of visible lesions, and reductions in the bacterial load and dissemination of infection. To reduce the cost of an oral vaccine and reduce the potential for environmental contamination with BCG, it is necessary to define the minimal efficacious dose of oral BCG for badgers. The objectives of the two studies reported here were to compare the efficacy of BCG Danish strain in a lipid matrix with unformulated BCG given orally, and to evaluate the efficacy of BCG Danish in a lipid matrix at a ten-fold lower dose than previously evaluated in badgers. In the first study, both BCG unformulated and in a lipid matrix reduced the number and apparent progression of visible lesions and the dissemination of infection from the lung. In the second study, vaccination with BCG in the lipid matrix at a ten-fold lower dose produced a similar outcome, but with greater intra-group variability than seen with the higher dose in the first study. Further research is needed before we are able to recommend a final dose of BCG for oral vaccination of badgers against TB or to know whether oral vaccination of wild badgers with BCG will significantly reduce transmission of the disease.

Item Type: Article
Subjects : Veterinary Medicine
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
NameEmailORCID
Chambers, MAUNSPECIFIEDUNSPECIFIED
Aldwell, FUNSPECIFIEDUNSPECIFIED
Williams, GAUNSPECIFIEDUNSPECIFIED
Palmer, SUNSPECIFIEDUNSPECIFIED
Gowtage, SUNSPECIFIEDUNSPECIFIED
Ashford, RUNSPECIFIEDUNSPECIFIED
Dalley, DUNSPECIFIEDUNSPECIFIED
Davé, DUNSPECIFIEDUNSPECIFIED
Weyer, UUNSPECIFIEDUNSPECIFIED
Salguero Bodes, Franciscof.salguerobodes@surrey.ac.ukUNSPECIFIED
Nunez, AUNSPECIFIEDUNSPECIFIED
Nadian, AUNSPECIFIEDUNSPECIFIED
Crawshaw, TUNSPECIFIEDUNSPECIFIED
Corner, LALUNSPECIFIEDUNSPECIFIED
Lesellier, SUNSPECIFIEDUNSPECIFIED
Date : 24 January 2017
Identification Number : 10.3389/fcimb.2017.00006
Copyright Disclaimer : Copyright © 2017 Chambers, Aldwell, Williams, Palmer, Gowtage, Ashford, Dalley, Davé, Weyer, Salguero, Nunez, Nadian, Crawshaw, Corner and Lesellier. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Uncontrolled Keywords : Badgers, Mycobacterium bovis, Oral BCG, Tuberculosis, Vaccine
Depositing User : Symplectic Elements
Date Deposited : 17 Jan 2017 15:32
Last Modified : 07 Jul 2017 10:56
URI: http://epubs.surrey.ac.uk/id/eprint/813305

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