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CD1- and CD1+ porcine blood dendritic cells are enriched for the orthologues of the two major mammalian conventional subsets

Edwards, JC, Everett, HE, Pedrera, M, Mokhtar, H, Marchi, E, Soldevila, F, Kaveh, DA, Hogarth, PJ, Johns, HL, Nunez-Garcia, J , Steinbach, Falko, Crooke, HR and Graham, Simon (2017) CD1- and CD1+ porcine blood dendritic cells are enriched for the orthologues of the two major mammalian conventional subsets Scientific Reports, 7, 40942.

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Abstract

Conventional dendritic cells (cDC) are professional antigen-presenting cells that induce immune activation or tolerance. Two functionally specialised populations, termed cDC1 and cDC2, have been described in humans, mice, ruminants and recently in pigs. Pigs are an important biomedical model species and a key source of animal protein; therefore further understanding of their immune system will help underpin the development of disease prevention strategies. To characterise cDC populations in porcine blood, DC were enriched from PBMC by CD14 depletion and CD172a enrichment then stained with a lineage mAbs (Lin; CD3, CD8α, CD14 and CD21) and mAbs specific for CD172a, CD1 and CD4. Two distinct porcine cDC subpopulations were FACSorted CD1- cDC (Lin-CD172+CD1-CD4-) and CD1+ cDC (Lin- CD172a+CD1+CD4-), and characterised by phenotypic and functional analyses. CD1+ cDC were distinct from CD1- cDC, expressing higher levels of CD172a, MHC class II and CD11b. Following TLR stimulation, CD1+ cDC produced IL-8 and IL-10 while CD1- cDC secreted IFN-α, IL-12 and TNF-α. CD1- cDC were superior in stimulating allogeneic T cell responses and in cross-presenting viral antigens to CD8 T cells. Comparison of transcriptional profiles further suggested that the CD1- and CD1+ populations were enriched for the orthologues of cDC1 and cDC2 subsets respectively.

Item Type: Article
Subjects : Veterinary Medicine
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
NameEmailORCID
Edwards, JCUNSPECIFIEDUNSPECIFIED
Everett, HEUNSPECIFIEDUNSPECIFIED
Pedrera, MUNSPECIFIEDUNSPECIFIED
Mokhtar, HUNSPECIFIEDUNSPECIFIED
Marchi, EUNSPECIFIEDUNSPECIFIED
Soldevila, FUNSPECIFIEDUNSPECIFIED
Kaveh, DAUNSPECIFIEDUNSPECIFIED
Hogarth, PJUNSPECIFIEDUNSPECIFIED
Johns, HLUNSPECIFIEDUNSPECIFIED
Nunez-Garcia, JUNSPECIFIEDUNSPECIFIED
Steinbach, Falkof.steinbach@surrey.ac.ukUNSPECIFIED
Crooke, HRUNSPECIFIEDUNSPECIFIED
Graham, Simons.graham@surrey.ac.ukUNSPECIFIED
Date : 20 January 2017
Identification Number : 10.1038/srep40942
Copyright Disclaimer : This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 04 Jan 2017 10:19
Last Modified : 07 Jul 2017 11:26
URI: http://epubs.surrey.ac.uk/id/eprint/813192

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