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Unmasking Ultradian Rhythms in Gene Expression

Van Der Veen, Daniel and Gerkema, MP (2016) Unmasking Ultradian Rhythms in Gene Expression The FASEB Journal, 31 (2). pp. 743-750.

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Abstract

Biological oscillations with an ultradian time scale of 1 to several hours include cycles in behavioral arousal, episodic glucocorticoid release, and gene expression. Ultradian rhythms are thought to have an extrinsic origin because of a perceived absence of ultradian rhythmicity in vitro and a lack of known molecular ultradian oscillators. We designed a novel, non–spectral-analysis method of separating ultradian from circadian components and applied it to a published gene expression dataset with an ultradian sampling resolution. Ultradian rhythms in mouse hepatocytes in vivo have been published, and we validated our approach using this control by confirming 175 of 323 ultradian genes identified in a prior study and found 862 additional ultradian genes. For the first time, we now report ultradian expression of >900 genes in vitro. Sixty genes exhibited ultradian transcriptional rhythmicity, both in vivo and in vitro, including 5 genes involved in the cell cycle. Within these 60 genes, we identified significant enrichment of specific DNA motifs in the 1000 bp proximal promotor, some of which associate with known transcriptional factors. These findings are in strong support of instrinsically-driven ultradian rhythms and expose potential molecular mechanisms and functions underlying ultradian rhythms that remain unknown.

Item Type: Article
Subjects : Biosciences and Medicine
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
NameEmailORCID
Van Der Veen, DanielD.Vanderveen@surrey.ac.ukUNSPECIFIED
Gerkema, MPUNSPECIFIEDUNSPECIFIED
Date : 8 November 2016
Identification Number : 10.1096/fj.201600872R
Copyright Disclaimer : Copyright 2016 The Author(s).This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) (http://creativecommons. org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Uncontrolled Keywords : biological rhythm • circadian • metabolism • cell culture • transcriptome
Depositing User : Symplectic Elements
Date Deposited : 22 Nov 2016 10:43
Last Modified : 22 Aug 2017 17:11
URI: http://epubs.surrey.ac.uk/id/eprint/812927

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