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L-fucose influences chemotaxis and biofilm formation in Campylobacter jejuni

Dwivedi, R, Nothaft, H, Garber, J, Xin Kin, L, Stahl, M, Flint, A, van Vliet, AHM, Stintzi, A and Szymanski, CM (2016) L-fucose influences chemotaxis and biofilm formation in Campylobacter jejuni Molecular Microbiology, 101 (4). pp. 575-589.

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Abstract

Campylobacter jejuni and Campylobacter coli are zoonotic pathogens once considered asaccharolytic, but are now known to encode pathways for glucose and fucose uptake/metabolism. For C. jejuni, strains with the fuc locus possess a competitive advantage in animal colonization models. We demonstrate that this locus is present in > 50% of genome-sequenced strains and is prevalent in livestock-associated isolates of both species. To better understand how these campylobacters sense nutrient availability, we examined biofilm formation and chemotaxis to fucose. C. jejuni NCTC11168 forms less biofilms in the presence of fucose, although its fucose permease mutant (fucP) shows no change. In a newly developed chemotaxis assay, both wild-type and the fucP mutant are chemotactic towards fucose. C. jejuni 81-176 naturally lacks the fuc locus and is unable to swim towards fucose. Transfer of the NCTC11168 locus into 81-176 activated fucose uptake and chemotaxis. Fucose chemotaxis also correlated with possession of the pathway for C. jejuni RM1221 (fuc+) and 81116 (fuc-). Systematic mutation of the NCTC11168 locus revealed that Cj0485 is necessary for fucose metabolism and chemotaxis. This study suggests that components for fucose chemotaxis are encoded within the fuc locus, but downstream signals only in fuc + strains, are involved in coordinating fucose availability with biofilm development.

Item Type: Article
Subjects : Veterinary Medicine
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
NameEmailORCID
Dwivedi, RUNSPECIFIEDUNSPECIFIED
Nothaft, HUNSPECIFIEDUNSPECIFIED
Garber, JUNSPECIFIEDUNSPECIFIED
Xin Kin, LUNSPECIFIEDUNSPECIFIED
Stahl, MUNSPECIFIEDUNSPECIFIED
Flint, AUNSPECIFIEDUNSPECIFIED
van Vliet, AHMUNSPECIFIEDUNSPECIFIED
Stintzi, AUNSPECIFIEDUNSPECIFIED
Szymanski, CMUNSPECIFIEDUNSPECIFIED
Date : 10 June 2016
Identification Number : https://doi.org/10.1111/mmi.13409
Copyright Disclaimer : This is the peer reviewed version of the following article: Dwivedi, R., Nothaft, H., Garber, J., Xin Kin, L., Stahl, M., Flint, A., van Vliet, A. H. M., Stintzi, A. and Szymanski, C. M. (2016), L-fucose influences chemotaxis and biofilm formation in Campylobacter jejuni. Molecular Microbiology, 101: 575–589. doi:10.1111/mmi.13409, which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1111/mmi.13409/full. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Depositing User : Symplectic Elements
Date Deposited : 17 Nov 2016 09:34
Last Modified : 10 Jun 2017 02:08
URI: http://epubs.surrey.ac.uk/id/eprint/812880

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