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Multilayer cellular stacks of gelatin-hydroxyapatite fiber scaffolds for bone tissue engineering

Salifu, A, Lekakou, Constantina and Labeed, Fatima (2016) Multilayer cellular stacks of gelatin-hydroxyapatite fiber scaffolds for bone tissue engineering Journal of Biomedical Materials Research: Part A, 105 (3). pp. 779-789.

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Abstract

Multilayer cellular stacks of crosslinked, electrospun 25 wt% hydroxyapatite (HA)-gelatin and pure gelatin fiber scaffolds, seeded with human foetal osteoblasts (hFOBs), were studied for up to 18 days in static and dynamic cell culture. Two types of stack models were investigated: a four-layer stack with cells seeded at the bottom surface of the first/top layer and the top surface of the fourth/bottom layer, so that the two middle layers were not seeded with cells with the aim to act as continuing conduits of culture medium and nutrients supply to the adjacent cell-populated zones; a three-layer stack with cells seeded at the bottom surface of each layer. hFOBs exhibited lower migration rate through the stack thickness for the 25 wt% HA-gelatin scaffolds compared to the pure gelatin scaffolds, due to the small pores of the former. Hence, the regularly seeded three-layer stack maintained cell-free porous zones in all layers through which the culture medium could continuously perfuse, while good fusion was achieved at the interface of all layers via the cross-migrating cells with a preference to downwards vertical migration attributed to gravity. Dynamic cell culture conditions enhanced overall cell growth by about 6% for the regularly seeded three-layer stack.

Item Type: Article
Subjects : Mechanical Engineering Science
Divisions : Faculty of Engineering and Physical Sciences > Mechanical Engineering Sciences
Authors :
NameEmailORCID
Salifu, AUNSPECIFIEDUNSPECIFIED
Lekakou, ConstantinaC.Lekakou@surrey.ac.ukUNSPECIFIED
Labeed, FatimaF.Labeed@surrey.ac.ukUNSPECIFIED
Date : 18 November 2016
Identification Number : 10.1002/jbm.a.35954
Copyright Disclaimer : This is the peer reviewed version of the following article: Salifu, A.A., Lekakou, C. and Labeed, F. (), Multilayer cellular stacks of gelatin-hydroxyapatite fiber scaffolds for bone tissue engineering. J. Biomed. Mater. Res.. Accepted Author Manuscript. doi:10.1002/jbm.a.35954, which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1002/jbm.a.35954/full. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving
Uncontrolled Keywords : Bone; Tissue engineering; stacks; natural scaffolds; electrospinning.
Depositing User : Symplectic Elements
Date Deposited : 02 Nov 2016 19:05
Last Modified : 07 Jul 2017 13:23
URI: http://epubs.surrey.ac.uk/id/eprint/812716

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