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The effect of long term exercise on intrahepatic lipid and the insulin sensitivity of glucose and fatty acid metabolism in sedentary male subjects

Shojaee-Moradie, F, Baynes, K, Pentecost, C, Bell, JD, Thomas, EL, Jackson, NC, Jackson, NC, Stolinski, M, Whyte, M, Jones, RH and Umpleby, AM (2004) The effect of long term exercise on intrahepatic lipid and the insulin sensitivity of glucose and fatty acid metabolism in sedentary male subjects In: 40th Annual Meeting of the European Association for the Study of Diabetes, 2004-09-05 - 2004-09-09, Munich, Germany.

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Abstract

Background and aims: Adaptive changes in fatty acid metabolism, liver and muscle fat content and adipocyte-derived cytokines may potentially explain the beneficial effects of exercise on insulin action. We investigated this in sedentary men before and after 6 weeks of supervised exercise. Materials and methods: Thirteen sedentary overweight male subjects (age 50 ± 304yr, BMI 28.2 ± 0.5) were recruited, seven were randomised to a 6 week exercise programme and six remained sedentary. After completion of the baseline (0 weeks) metabolic study and body composition measurements subjects who were allocated to the exercise group started the exercise programme. Subjects exercised at 60-85% ofV02 max for a minimum of 20 minutes at least 3 times a week for 6 weeks. Insulin sensitivity of fatty acid (NEFA) production rate (Ra), glycerol Ra, glucose Ra and glucose disposal rate (Rd) were measured with stable isotopes of palmitic acid, glycerol and glucose at 0 and 6 weeks with a 2 step hyperinsulinaemic euglycaemic clamp (step 1, 0.3 (low dose); step 2, 1.5 (high dose) mU. kg-I. min-I). Intrahepatocellular lipid (IHCL) and intramyocellular lipid (IMCL) were measured by magnetic resonance spectroscopy and visceral fat by cross-sectional CT scanning. Results: In the exercise group V02 max increased by 20 ± 5% after 6 weeks (p<O.Ol) but there was no significant change in the control group. There was no significant change in body weight or BMI in either group. After 6 weeks, the decrease in glucose Ra following low dose insulin was greater than at 0 week in the exercise group (3.8 ± 0.5 vs 2.8 ± O.5llmol.kg-l.min-t, 0 week vs 6 weeks, p<0.05) with no change in the control group (3.2 ± 0.7 vs 3.2±004llmol.kg-l.min-I). After 6 weeks the increase in the area under the curve for glucose Rd following high dose insulin was greater than at 0 week in the exercise group (5404 ± 7351lmol!kg vs 6508 ± 581llmol!kg, p<O.Ol) with no change in the control group (4664 ± 5381lffiol!kg vs 4628 ± 415 Ilmol!kg). In the exercise group there was a decrease in fasting NEFA concentration (0.81 ±0.04 vs 0.62 ± 0.05 mmol!l, 0 week vs 6 weeks, respectively, p<O.Ol), fasting glycerol concentration (75 ± 8.3 vs 58 ± 6.6Ilffiol!l, p<0.03), fasting glycerol Ra (3.3±0.8 vs 2.5±0.6Ilmol· kg-I. min-l ,p<0.02) and fasting NEFA Ra (4.5 ± 0.3 vs 3.7 ± 0.51lmol . kg-I . min-I, p<0.04), visceral fat content (186 ± 13 cm2 vs 152 ± 10 cm2, p<0.04) and fasting adiponectin (7.0 ± 0.91lglml vs 5.8 ± 0.9Ilg/ml, p<0.05) but no change in the control group. There was no change in IMCL in either group. The improved insulin sensitivity of glucose Ra in the exercise group correlated with a decrease in IHCL (p<0.05). Conclusion: The improvement in the insulin sensitivity of lipolysis, results in decreased availability of circulating NEFA which in some subjects may lead to a reduction in IHCL content. This may contribute to the observed improvement in the insulin sensitivity of glucose Ra with exercise.

Item Type: Conference or Workshop Item (Conference Poster)
Subjects : Nutrition
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine > Department of Nutritional Sciences
Authors :
AuthorsEmailORCID
Shojaee-Moradie, FUNSPECIFIEDUNSPECIFIED
Baynes, KUNSPECIFIEDUNSPECIFIED
Pentecost, CUNSPECIFIEDUNSPECIFIED
Bell, JDUNSPECIFIEDUNSPECIFIED
Thomas, ELUNSPECIFIEDUNSPECIFIED
Jackson, NCUNSPECIFIEDUNSPECIFIED
Jackson, NCUNSPECIFIEDUNSPECIFIED
Stolinski, MUNSPECIFIEDUNSPECIFIED
Whyte, MUNSPECIFIEDUNSPECIFIED
Jones, RHUNSPECIFIEDUNSPECIFIED
Umpleby, AMUNSPECIFIEDUNSPECIFIED
Date : 1 August 2004
Identification Number : https://doi.org/10.1007/BF03375463
Copyright Disclaimer : © Springer-Verlag 2004
Uncontrolled Keywords : Science & Technology, Life Sciences & Biomedicine, Endocrinology & Metabolism
Depositing User : Symplectic Elements
Date Deposited : 24 Oct 2016 09:51
Last Modified : 24 Oct 2016 09:51
URI: http://epubs.surrey.ac.uk/id/eprint/812561

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