University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Accurate quantification of apoptosis progression and toxicity using a dielectrophoretic approach

Henslee, EA, Torcal Serrano, RM, Labeed, FH, Jabr, RI, Fry, CH, Hughes, MP and Hoettges, KF (2016) Accurate quantification of apoptosis progression and toxicity using a dielectrophoretic approach Analyst, 141. pp. 6408-6415.

[img]
Preview
Text
C6AN01596D.pdf - Version of Record
Available under License : See the attached licence file.

Download (427kB) | Preview
[img]
Preview
Text (licence)
SRI_deposit_agreement.pdf
Available under License : See the attached licence file.

Download (33kB) | Preview
[img] Text
AnalystHenslee et al REVISED clean version.docx - Accepted version Manuscript
Restricted to Repository staff only
Available under License : See the attached licence file.

Download (4MB)

Abstract

A loss of ability of cells to undergo apoptosis (programmed cell death, whereby the cell ceases to function and destroys itself) is commonly associated with cancer, and many anti-cancer interventions aim to restart the process. Consequently, the accurate quantification of apoptosis is essential in understanding the function and performance of new anti-cancer drugs. Dielectrophoresis has previously been demonstrated to detect apoptosis more rapidly than other methods, and is low-cost, label-free and rapid, but has previously been unable to accurately quantify cells through the apoptotic process because cells in late apoptosis disintegrate, making cell tracking impossible. In this paper we use a novel method based on light absorbance and multi-population tracking to quantify the progress of apoptosis, benchmarking against conventional assays including MTT, Trypan Blue and Annexin-V. Analyses are performed on suspension and adherent cells, and using two apoptosis-inducing agents. IC50 measurements compared favourably to MTT and were superior to Trypan Blue, whilst also detecting apoptotic progression faster than Annexin-V.

Item Type: Article
Subjects : Mechanical Engineering
Divisions : Faculty of Engineering and Physical Sciences > Mechanical Engineering Sciences
Authors :
AuthorsEmailORCID
Henslee, EAUNSPECIFIEDUNSPECIFIED
Torcal Serrano, RMUNSPECIFIEDUNSPECIFIED
Labeed, FHUNSPECIFIEDUNSPECIFIED
Jabr, RIUNSPECIFIEDUNSPECIFIED
Fry, CHUNSPECIFIEDUNSPECIFIED
Hughes, MPUNSPECIFIEDUNSPECIFIED
Hoettges, KFUNSPECIFIEDUNSPECIFIED
Date : 19 October 2016
Identification Number : https://doi.org/10.1039/c6an01596d
Copyright Disclaimer : This Open Access Article is licensed under a Creative Commons Attribution-Non Commercial 3.0 Unported Licence
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 19 Oct 2016 13:38
Last Modified : 06 Dec 2016 16:01
URI: http://epubs.surrey.ac.uk/id/eprint/812506

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800