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Pericyte seeded dual peptide scaffold with improved endothelialization for vascular graft tissue engineering

Campagnolo, P, Gormley, AJ, Chow, LW, Guex, AG, Parmar, PA, Puetzer, JL, Steele, JAM, Breant, A, Madeddu, P and Stevens, MM (2016) Pericyte seeded dual peptide scaffold with improved endothelialization for vascular graft tissue engineering Advanced healthcare materials.

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Abstract

The development of synthetic vascular grafts for coronary artery bypass is challenged by insufficient endothelialization, which exposes to the risk of thrombosis, and lack of native cellular constituents, which favours pathological remodelling. Here, an bifunctional electrospun poly(ε-caprolactone) (PCL) scaffold with potential for synthetic vascular graft applications is presented. This scaffold incorporates two tethered peptides: the osteopontin-derived peptide (Adh) on the ‘luminal’ side and a heparin-binding peptide (Hep) on the ‘abluminal’ side. Additionally, the ‘abluminal’ side of the scaffold is seeded with saphenous vein-derived pericytes (SVPs) as a source of pro-angiogenic growth factors. The Adh peptide significantly increase endothelial cell adhesion, while the Hep peptide promote accumulation of vascular endothelial growth factor (VEGF) secreted by SVPs. SVPs increase endothelial migration both in a transwell assay and a modified scratch assay performed on the PCL scaffold. Seeding of SVPs on the ‘abluminal’/Hep side of the scaffold further increase endothelial cell density, indicating a combinatory effect of the peptides and pericytes. Lastly, SVP-seeded scaffolds are preserved by freezing in a xeno-free medium, maintaining good cell viability and function. In conclusion, this engineered scaffold combines patient-derived pericytes and spatially organized functionalities, which synergistically increase endothelial cell density and growth factor retention.

Item Type: Article
Subjects : Medical Science
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
AuthorsEmailORCID
Campagnolo, PUNSPECIFIEDUNSPECIFIED
Gormley, AJUNSPECIFIEDUNSPECIFIED
Chow, LWUNSPECIFIEDUNSPECIFIED
Guex, AGUNSPECIFIEDUNSPECIFIED
Parmar, PAUNSPECIFIEDUNSPECIFIED
Puetzer, JLUNSPECIFIEDUNSPECIFIED
Steele, JAMUNSPECIFIEDUNSPECIFIED
Breant, AUNSPECIFIEDUNSPECIFIED
Madeddu, PUNSPECIFIEDUNSPECIFIED
Stevens, MMUNSPECIFIEDUNSPECIFIED
Date : 26 October 2016
Identification Number : https://doi.org/10.1002/adhm.201600699
Copyright Disclaimer : This is the peer reviewed version of the following article: Campagnolo P, Gormley AJ, Chow LW, Guex AG, Parmar PA, Puetzer JL, Steele JAM, Breant A, Madeddu P, Stevens MM, Pericyte seeded dual peptide scaffold with improved endothelialization for vascular graft tissue engineering which has been published in final form at http://doi.org/10.1002/adhm.201600699 This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 Oct 2016 09:44
Last Modified : 04 Nov 2016 14:46
URI: http://epubs.surrey.ac.uk/id/eprint/812469

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