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Detection of candidate biomarkers of prostate cancer progression in serum: a depletion-free 3D LC/MS quantitative proteomics pilot study

Larkin, SET, Johnston, HE, Jackson, TR, Jamieson, DG, Roumeliotis, TI, Mockridge, CI, Manousopoulou, A, Papachristou, EK, Brown, MD, Clarke, NW , Pandha, HS, Aukim-Hastie, CL, Cragg, MS, Garbis, SD and Townsend, PA (2016) Detection of candidate biomarkers of prostate cancer progression in serum: a depletion-free 3D LC/MS quantitative proteomics pilot study British Journal of Cancer.

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Abstract

Background: Prostate cancer (PCa) is the most common male cancer in the United Kingdom and we aimed to identify clinically relevant biomarkers corresponding to stage progression of the disease. Methods: We used enhanced proteomic profiling of PCa progression using iTRAQ 3D LC mass spectrometry on high-quality serum samples to identify biomarkers of PCa. Results: We identified >1000 proteins. Following specific inclusion/exclusion criteria we targeted seven proteins of which two were validated by ELISA and six potentially interacted forming an ‘interactome’ with only a single protein linking each marker. This network also includes accepted cancer markers, such as TNF, STAT3, NF-κB and IL6. Conclusions: Our linked and interrelated biomarker network highlights the potential utility of six of our seven markers as a panel for diagnosing PCa and, critically, in determining the stage of the disease. Our validation analysis of the MS-identified proteins found that SAA alongside KLK3 may improve categorisation of PCa than by KLK3 alone, and that TSR1, although not significant in this model, might also be a clinically relevant biomarker.

Item Type: Article
Subjects : Medical Science
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
NameEmailORCID
Larkin, SETUNSPECIFIEDUNSPECIFIED
Johnston, HEUNSPECIFIEDUNSPECIFIED
Jackson, TRUNSPECIFIEDUNSPECIFIED
Jamieson, DGUNSPECIFIEDUNSPECIFIED
Roumeliotis, TIUNSPECIFIEDUNSPECIFIED
Mockridge, CIUNSPECIFIEDUNSPECIFIED
Manousopoulou, AUNSPECIFIEDUNSPECIFIED
Papachristou, EKUNSPECIFIEDUNSPECIFIED
Brown, MDUNSPECIFIEDUNSPECIFIED
Clarke, NWUNSPECIFIEDUNSPECIFIED
Pandha, HSUNSPECIFIEDUNSPECIFIED
Aukim-Hastie, CLUNSPECIFIEDUNSPECIFIED
Cragg, MSUNSPECIFIEDUNSPECIFIED
Garbis, SDUNSPECIFIEDUNSPECIFIED
Townsend, PAUNSPECIFIEDUNSPECIFIED
Date : 29 September 2016
Identification Number : 10.1038/bjc.2016.291
Copyright Disclaimer : Copyright 2016 Cancer Research UK.This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Uncontrolled Keywords : Prostate Cancer, Proteomics, PSA, LCMS, iTRAQ
Depositing User : Symplectic Elements
Date Deposited : 10 Oct 2016 13:58
Last Modified : 10 Oct 2016 13:58
URI: http://epubs.surrey.ac.uk/id/eprint/812356

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