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Automated Microbeam Observation Environment for Biological Analysis − Custom Portable Environmental Control applied to a Vertical Microbeam System

England, MJ, Bigelow, AW, Merchant, MJ, Velliou, E, Welch, D, Brenner, DJ and Kirkby, KJ (2016) Automated Microbeam Observation Environment for Biological Analysis − Custom Portable Environmental Control applied to a Vertical Microbeam System Sensors and Actuators B: Chemical, 239. pp. 1134-1143.

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Abstract

Vertical Microbeams (VMB) are used to irradiate individual cells with low MeV energy ions. The irradiation of cells using VMBs requires cells to be removed from an incubator; this can cause physiological changes to cells because of the lower CO2 concentration, temperature and relative humidity outside of the incubator. Consequently, for experiments where cells require irradiation and observation for extended time periods, it is important to provide a controlled environment. The highly customised nature of the microscopes used on VMB systems means that there are no commercially available environmentally controlled microscope systems for VMB systems. The Automated Microbeam Observation Environment for Biological Analysis (AMOEBA) is a highly flexible modular environmental control system used to create incubator conditions on the end of a VMB. The AMOEBA takes advantage of the recent “maker” movement to create an open source control system that can be easily configured by the user to fit their control needs even beyond VMB applications. When applied to the task of controlling cell medium temperature, CO2 concentration and relative humidity on VMBs it creates a stable environment that allows cells to multiply on the end of a VMB over a period of 36 hours, providing a low-cost (costing less than $2700 to build), customisable alternative to commercial time-lapse microscopy systems. AMOEBA adds the potential of VMBs to explore the long-term effects of radiation on single cells opening up new research areas for VMBs.

Item Type: Article
Subjects : Chemical & Process Engineering
Divisions : Faculty of Engineering and Physical Sciences > Chemical and Process Engineering
Authors :
AuthorsEmailORCID
England, MJUNSPECIFIEDUNSPECIFIED
Bigelow, AWUNSPECIFIEDUNSPECIFIED
Merchant, MJUNSPECIFIEDUNSPECIFIED
Velliou, EUNSPECIFIEDUNSPECIFIED
Welch, DUNSPECIFIEDUNSPECIFIED
Brenner, DJUNSPECIFIEDUNSPECIFIED
Kirkby, KJUNSPECIFIEDUNSPECIFIED
Date : 16 August 2016
Identification Number : 10.1016/j.snb.2016.08.076
Copyright Disclaimer : © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Uncontrolled Keywords : Microbeam; Time-lapse microscopy; environmental control; Arduino; 3D printing,
Depositing User : Symplectic Elements
Date Deposited : 26 Aug 2016 11:22
Last Modified : 22 Nov 2016 17:15
URI: http://epubs.surrey.ac.uk/id/eprint/811881

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