Direct quantification of rare earth doped titania nanoparticles in individual human cells
Palitsin, V, Jeynes, JCG, Jeynes, C and Townley, HE (2016) Direct quantification of rare earth doped titania nanoparticles in individual human cells Nanotechnology, 27 (28).
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There are many possible biomedical applications for titania nanoparticles (NPs) doped with rare earth elements (REEs), from dose enhancement and diagnostic imaging in radiotherapy, to biosensing. However, there are concerns that the NPs could disintegrate in the body thus releasing toxic REE ions to undesired locations. As a first step, we investigate how accurately the Ti/REE ratio from the NPs can be measured inside human cells. A quantitative analysis of whole, unsectioned, individual human cells was performed using proton microprobe elemental microscopy. This method is unique in being able to quantitatively analyse all the elements in an unsectioned individual cell with micron resolution, while also scanning large fields of view. We compared the Ti/REE signal inside cells to NPs that were outside the cells, non-specifically absorbed onto the polypropylene substrate. We show that the REE signal in individual cells co-localises with the titanium signal, indicating that the NPs have remained intact. Within the uncertainty of the measurement, there is no difference between the Ti/REE ratio inside and outside the cells. Interestingly, we also show that there is considerable variation in the uptake of the NPs from cell-to-cell, by a factor of more than 10. We conclude that the NPs enter the cells and remain intact. The large heterogeneity in NP concentrations from cell-to-cell should be considered if they are to be used therapeutically.
|Subjects :||Electronic Engineering|
|Divisions :||Faculty of Engineering and Physical Sciences > Electronic Engineering > Advanced Technology Institute > Ion Beam Centre|
|Date :||3 June 2016|
|Identification Number :||https://doi.org/10.1088/0957-4484/27/28/285103|
|Copyright Disclaimer :||Original content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.|
|Uncontrolled Keywords :||ion beam analysis, proton induced x-ray emission, lanthanides, nanoparticles, human cells|
|Depositing User :||Symplectic Elements|
|Date Deposited :||06 Sep 2016 14:17|
|Last Modified :||06 Sep 2016 14:17|
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