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Properties of alternative microbial hosts used in Synthetic Biology: Towards the design of a modular chassis

Kim, J, Salvador, M, Saunders, E, González, J, Avignone-Rossa, C and Jimenez, JI (2016) Properties of alternative microbial hosts used in Synthetic Biology: Towards the design of a modular chassis Essays in Biochemistry.

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Abstract

The chassis is the cellular host used as a recipient of engineered biological systems in synthetic biology. They are required to propagate the genetic information and to express the genes encoded in it. Despite being an essential element for the appropriate function of genetic circuits, the chassis is rarely considered in their design phase. Consequently, the circuits are transferred to model organisms commonly used in the lab, such as Escherichia coli, that may be suboptimal for a required function. In this review we discuss some of the properties desirable in a versatile chassis and summarize some examples of alternative hosts for synthetic biology amenable for engineering. These properties include a suitable life style, a robust cell wall, good knowledge of its regulatory network as well as of the interplay of the host components with the exogenous circuits, the possibility of developing whole-cell models and tunable metabolic fluxes that could allow a better distribution of cellular resources (metabolites, ATP, nucleotides, amino acids, transcriptional and translational machinery). We highlight Pseudomonas putida, widely used in many different biotechnological applications as a prominent organism for synthetic biology due to its metabolic diversity, robustness and ease of manipulation.

Item Type: Article
Subjects : Biosciences
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
AuthorsEmailORCID
Kim, JUNSPECIFIEDUNSPECIFIED
Salvador, MUNSPECIFIEDUNSPECIFIED
Saunders, EUNSPECIFIEDUNSPECIFIED
González, JUNSPECIFIEDUNSPECIFIED
Avignone-Rossa, CUNSPECIFIEDUNSPECIFIED
Jimenez, JIUNSPECIFIEDUNSPECIFIED
Date : 2016
Funders : BBSRC
Copyright Disclaimer : This version is the authors accepted manuscript. The version of record will be available from http://essays.biochemistry.org/
Depositing User : Symplectic Elements
Date Deposited : 10 Aug 2016 09:14
Last Modified : 10 Aug 2016 09:14
URI: http://epubs.surrey.ac.uk/id/eprint/811650

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