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Properties of alternative microbial hosts used in Synthetic Biology: Towards the design of a modular chassis

Kim, Juhyun, Salvador de Lara, Manuel, Saunders, Elizabeth, Gonzalez Gutierrez De La Conch, Jaime, Avignone Rossa, Claudio and Jimenez, Jose (2016) Properties of alternative microbial hosts used in Synthetic Biology: Towards the design of a modular chassis Essays in Biochemistry, 60 (4). pp. 303-313.

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The chassis is the cellular host used as a recipient of engineered biological systems in synthetic biology. They are required to propagate the genetic information and to express the genes encoded in it. Despite being an essential element for the appropriate function of genetic circuits, the chassis is rarely considered in their design phase. Consequently, the circuits are transferred to model organisms commonly used in the lab, such as Escherichia coli, that may be suboptimal for a required function. In this review we discuss some of the properties desirable in a versatile chassis and summarize some examples of alternative hosts for synthetic biology amenable for engineering. These properties include a suitable life style, a robust cell wall, good knowledge of its regulatory network as well as of the interplay of the host components with the exogenous circuits, the possibility of developing whole-cell models and tunable metabolic fluxes that could allow a better distribution of cellular resources (metabolites, ATP, nucleotides, amino acids, transcriptional and translational machinery). We highlight Pseudomonas putida, widely used in many different biotechnological applications as a prominent organism for synthetic biology due to its metabolic diversity, robustness and ease of manipulation.

Item Type: Article
Subjects : Biosciences
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
Salvador de Lara,
Gonzalez Gutierrez De La Conch,
Avignone Rossa,
Date : 30 November 2016
Funders : Biotechnology and Biological Sciences Research Council (BBSRC)
DOI : 10.1042/EBC20160015
Copyright Disclaimer : © 2016 The Author(s) This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution Licence 4.0 (CC BY).
Depositing User : Symplectic Elements
Date Deposited : 10 Aug 2016 09:14
Last Modified : 11 Dec 2018 11:22

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