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Optimal determination of steric mass action model parameters for beta-lactoglobulin using static batch experiments.

Barz, T, Löffler, V, Arellano-Garcia, H and Wozny, G (2010) Optimal determination of steric mass action model parameters for beta-lactoglobulin using static batch experiments. J Chromatogr A, 1217 (26). pp. 4267-4277.

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Abstract

In this work, parameters of the steric mass-formalism SMA are optimally ascertained for a reliable determination of the adsorption isotherms of beta-lactoglobulin A and B under non-isocratic conditions. For this purpose, static batch experiments are used in contrast to the protocols based on different experimental steps, which use a chromatographic column. It is shown that parameters can already be determined for a small number of experiments by using a systematic procedure based on optimal model-based experimental design and an efficient NLP-solver. The in different works observed anti-Langmuir shape of the isotherm for small concentrations of beta-lactoglobulin A was corroborated. Moreover, we also found indications for a porosity variation with changing protein concentrations.

Item Type: Article
Authors :
AuthorsEmailORCID
Barz, TUNSPECIFIEDUNSPECIFIED
Löffler, VUNSPECIFIEDUNSPECIFIED
Arellano-Garcia, HUNSPECIFIEDUNSPECIFIED
Wozny, GUNSPECIFIEDUNSPECIFIED
Date : 25 June 2010
Identification Number : https://doi.org/10.1016/j.chroma.2010.04.001
Uncontrolled Keywords : Adsorption, Chromatography, Ion Exchange, Lactoglobulins, Models, Theoretical, Static Electricity, Stereoisomerism, Thermodynamics
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 28 Mar 2017 15:32
Last Modified : 28 Mar 2017 15:32
URI: http://epubs.surrey.ac.uk/id/eprint/811532

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