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Mapping polyclonal antibody responses to bacterial infection using next generation phage display

Naqid, IA, Owen, JP, Maddison, BC, Spiliotopoulos, A, Emes, RD, Warry, A, Tchórzewska, MA, Martelli, F, Gosling, RJ, Davies, RH, La Ragione, RM and Gough, KC (2016) Mapping polyclonal antibody responses to bacterial infection using next generation phage display Scientific Reports, 6, 24232.

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Abstract

Mapping polyclonal antibody responses to infectious diseases to identify individual epitopes has the potential to underpin the development of novel serological assays and vaccines. Here, phage-peptide library panning coupled with screening using next generation sequencing was used to map antibody responses to bacterial infections. In the first instance, pigs experimentally infected with Salmonella enterica serovar Typhimurium was investigated. IgG samples from twelve infected pigs were probed in parallel and phage binding compared to that with equivalent IgG from pre-infected animals. Seventy-seven peptide mimotopes were enriched specifically against sera from multiple infected animals. Twenty-seven of these peptides were tested in ELISA and twenty-two were highly discriminatory for sera taken from pigs post-infection (P < 0.05) indicating that these peptides are mimicking epitopes from the bacteria. In order to further test this methodology, it was applied to differentiate antibody responses in poultry to infections with distinct serovars of Salmonella enterica. Twenty-seven peptides were identified as being enriched specifically against IgY from multiple animals infected with S. Enteritidis compared to those infected with S. Hadar. Nine of fifteen peptides tested in ELISA were highly discriminatory for IgY following S. Enteritidis infection (p < 0.05) compared to infections with S. Hadar or S. Typhimurium.

Item Type: Article
Subjects : Veterinary Medicine
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
AuthorsEmailORCID
Naqid, IAUNSPECIFIEDUNSPECIFIED
Owen, JPUNSPECIFIEDUNSPECIFIED
Maddison, BCUNSPECIFIEDUNSPECIFIED
Spiliotopoulos, AUNSPECIFIEDUNSPECIFIED
Emes, RDUNSPECIFIEDUNSPECIFIED
Warry, AUNSPECIFIEDUNSPECIFIED
Tchórzewska, MAUNSPECIFIEDUNSPECIFIED
Martelli, FUNSPECIFIEDUNSPECIFIED
Gosling, RJUNSPECIFIEDUNSPECIFIED
Davies, RHUNSPECIFIEDUNSPECIFIED
La Ragione, RMUNSPECIFIEDUNSPECIFIED
Gough, KCUNSPECIFIEDUNSPECIFIED
Date : 13 April 2016
Identification Number : 10.1038/srep24232
Copyright Disclaimer : This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Depositing User : Symplectic Elements
Date Deposited : 19 Jul 2016 11:23
Last Modified : 19 Jul 2016 11:23
URI: http://epubs.surrey.ac.uk/id/eprint/811254

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