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Limited genetic and antigenic diversity within parasite isolates used in a live vaccine against Theileria parva

Hemmink, JD, Weir, W, MacHugh, ND, Graham, SP, Patel, E, Paxton, E, Shiels, B, Toye, PG, Morrison, WI and Pelle, R (2016) Limited genetic and antigenic diversity within parasite isolates used in a live vaccine against Theileria parva International Journal for Parasitology, 46 (8). pp. 495-506.

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Abstract

An infection and treatment protocol is used to vaccinate cattle against Theileria parva infection. Due to incomplete cross-protection between different parasite isolates, a mixture of three isolates, termed the Muguga cocktail, is used for vaccination. While vaccination of cattle in some regions provides high levels of protection, some animals are not protected against challenge with buffalo-derived T. parva. Knowledge of the genetic composition of the Muguga cocktail vaccine is required to understand how vaccination is able to protect against field challenge and to identify the potential limitations of the vaccine. The aim of the current study was to determine the extent of genetic and antigenic diversity within the parasite isolates that constitute the Muguga cocktail. High throughput multi-locus sequencing of antigen-encoding loci was performed in parallel with typing using a panel of micro- and minisatellite loci. The former focused on genes encoding CD8+ T cell antigens, believed to be relevant to protective immunity. The results demonstrate that each of the three component stocks of the cocktail contains limited parasite genotypic diversity, with single alleles detected at many gene/satellite loci and, moreover, that two of the components show a very high level of similarity. Thus, the vaccine incorporates very little of the genetic and antigenic diversity observed in field populations of T. parva. The presence of alleles at low frequency (<10%) within vaccine component populations also points to the possibility of variability in the content of vaccine doses and the potential for loss of allelic diversity during tick passage. The results demonstrate that there is scope to modify the content of the vaccine in order to enhance its diversity and thus its potential for providing broad protection. The ability to accurately quantify genetic diversity in vaccine component stocks will facilitate improved quality control procedures designed to ensure the long-term efficacy of the vaccine.

Item Type: Article
Subjects : Veterinary Medicine
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
AuthorsEmailORCID
Hemmink, JDUNSPECIFIEDUNSPECIFIED
Weir, WUNSPECIFIEDUNSPECIFIED
MacHugh, NDUNSPECIFIEDUNSPECIFIED
Graham, SPUNSPECIFIEDUNSPECIFIED
Patel, EUNSPECIFIEDUNSPECIFIED
Paxton, EUNSPECIFIEDUNSPECIFIED
Shiels, BUNSPECIFIEDUNSPECIFIED
Toye, PGUNSPECIFIEDUNSPECIFIED
Morrison, WIUNSPECIFIEDUNSPECIFIED
Pelle, RUNSPECIFIEDUNSPECIFIED
Date : July 2016
Funders : Biotechnology and Biological Sciences Research Council
Identification Number : 10.1016/j.ijpara.2016.02.007
Copyright Disclaimer : © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Uncontrolled Keywords : Theileria parva, Vaccination, Cattle, Antigenic diversity, Gene sequencing, Satellite DNA
Depositing User : Symplectic Elements
Date Deposited : 08 Jul 2016 14:32
Last Modified : 08 Jul 2016 14:32
URI: http://epubs.surrey.ac.uk/id/eprint/811149

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