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Immunoglobulin light chain allelic inclusion in systemic lupus erythematosus

Fraser, LD, Zhao, Y, Lutalo, PMK, D’Cruz, DP, Cason, J, Silva, JS, Dunn-Walters, D, Nayar, S, Cope, AP and Spencer, J (2016) Immunoglobulin light chain allelic inclusion in systemic lupus erythematosus European Journal of Immunology, 45 (8). pp. 2409-2419.

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Abstract

The principles of allelic exclusion state that each B cell expresses a single light and heavy chain pair. Here, we show that B cells with both kappa and lambda light chains (Igκ and Igλ) are enriched in some patients with the systemic autoimmune disease systemic lupus erythematosus (SLE), but not in the systemic autoimmune disease control granulomatosis with polyangiitis. Detection of dual Igκ and Igλ expression by flow cytometry could not be abolished by acid washing or by DNAse treatment to remove any bound polyclonal antibody or complexes, and was retained after two days in culture. Both surface and intracytoplasmic dual light chain expression was evident by flow cytometry and confocal microscopy. We observed reduced frequency of rearrangements of the kappa-deleting element (KDE) in SLE and an inverse correlation between the frequency of KDE rearrangement and the frequency of dual light chain expressing B cells. We propose that dual expression of Igκ and Igλ by a single B cell may occur in some patients with SLE when this may be a consequence of reduced activity of the KDE.

Item Type: Article
Subjects : Biosciences and Medicine
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
AuthorsEmailORCID
Fraser, LDUNSPECIFIEDUNSPECIFIED
Zhao, YUNSPECIFIEDUNSPECIFIED
Lutalo, PMKUNSPECIFIEDUNSPECIFIED
D’Cruz, DPUNSPECIFIEDUNSPECIFIED
Cason, JUNSPECIFIEDUNSPECIFIED
Silva, JSUNSPECIFIEDUNSPECIFIED
Dunn-Walters, DUNSPECIFIEDUNSPECIFIED
Nayar, SUNSPECIFIEDUNSPECIFIED
Cope, APUNSPECIFIEDUNSPECIFIED
Spencer, JUNSPECIFIEDUNSPECIFIED
Date : 24 June 2016
Identification Number : 10.1002/eji.201545599
Copyright Disclaimer : © 2015 The Authors. European Journal of Immunology published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.*** This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Uncontrolled Keywords : Allelic inclusion;Immunoglobulin;Igκ;Igλ;Light chain;Systemic lupus erythematosus
Depositing User : Symplectic Elements
Date Deposited : 06 Jul 2016 14:07
Last Modified : 06 Jul 2016 14:07
URI: http://epubs.surrey.ac.uk/id/eprint/811130

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