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Exercise duration-matched interval and continuous sprint cycling induce similar increases in AMPK phosphorylation, PGC-1α and VEGF mRNA expression in trained individuals

Taylor, CW, Ingham, SA, Hunt, JEA, Martin, NRW, Pringle, JSM and Ferguson, RA (2016) Exercise duration-matched interval and continuous sprint cycling induce similar increases in AMPK phosphorylation, PGC-1α and VEGF mRNA expression in trained individuals European Journal of Applied Physiology.

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Abstract

Purpose The effects of low-volume interval and continuous ‘all-out’ cycling, matched for total exercise duration, on mitochondrial and angiogenic cell signalling was investigated in trained individuals. Methods In a repeated measures design, 8 trained males (\(\dot{V}{\text{O}}_{{2{\text{peak}}}}\), 57 ± 7 ml kg−1 min−1) performed two cycling exercise protocols; interval (INT, 4 × 30 s maximal sprints interspersed by 4 min passive recovery) or continuous (CON, 2 min continuous maximal sprint). Muscle biopsies were obtained before, immediately after and 3 h post-exercise. Results Total work was 53 % greater (P = 0.01) in INT compared to CON (71.2 ± 7.3 vs. 46.3 ± 2.7 kJ, respectively). Phosphorylation of AMPKThr172 increased by a similar magnitude (P = 0.347) immediately post INT and CON (1.6 ± 0.2 and 1.3 ± 0.3 fold, respectively; P = 0.011), before returning to resting values at 3 h post-exercise. mRNA expression of PGC-1α (7.1 ± 2.1 vs. 5.5 ± 1.8 fold; P = 0.007), VEGF (3.5 ± 1.2 vs. 4.3 ± 1.8 fold; P = 0.02) and HIF-1α (2.0 ± 0.5 vs. 1.5 ± 0.3 fold; P = 0.04) increased at 3 h post-exercise in response to INT and CON, respectively; the magnitude of which were not different between protocols. Conclusions Despite differences in total work done, low-volume INT and CON ‘all-out’ cycling, matched for exercise duration, provides a similar stimulus for the induction of mitochondrial and angiogenic cell signalling pathways in trained skeletal muscle.

Item Type: Article
Subjects : Biosciences
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
AuthorsEmailORCID
Taylor, CWUNSPECIFIEDUNSPECIFIED
Ingham, SAUNSPECIFIEDUNSPECIFIED
Hunt, JEAUNSPECIFIEDUNSPECIFIED
Martin, NRWUNSPECIFIEDUNSPECIFIED
Pringle, JSMUNSPECIFIEDUNSPECIFIED
Ferguson, RAUNSPECIFIEDUNSPECIFIED
Date : 1 June 2016
Identification Number : 10.1007/s00421-016-3402-2
Copyright Disclaimer : © The Author(s) 2016. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 05 Jul 2016 13:46
Last Modified : 05 Jul 2016 13:46
URI: http://epubs.surrey.ac.uk/id/eprint/811112

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