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Double Thermoresponsive Block Copolymers Featuring a Biotin End Group

Jochum, FD, Roth, PJ, Kessler, D and Theato, P (2010) Double Thermoresponsive Block Copolymers Featuring a Biotin End Group BIOMACROMOLECULES, 11 (9). pp. 2432-2439.

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Abstract

A poly(oligo(ethylene glycol) monomethyl ether methacrylate)-block-poly(N-isopropyl methacrylamide) (POEGMAb-PNIPMAM) block copolymer with a biotin end group on the PNIPMAM block as a biotarget was synthesized as a model system for temperature-controlled polymer immobilization. The synthesis was based on RAFT polymerization followed by postpolymerization modification of an activated ester precursor block and an exchange of the dithioester end group within one step. NMR, differential scanning calorimetry (DSC), dynamic light scattering (DLS), and turbidimetry measurements were performed to investigate the stimulus-responsive properties. The double thermoresponsive POEGMA-b-PNIPMAM with biotin end group showed a temperature-dependent multistage assembly behavior as it was completely soluble in water at temperatures below the LCST of both blocks, formed micellar structures above the LCST of PNIPMAM but below the LCST of POEGMA, or precipitated from solution above the LCST of both blocks. At room temperature, the polymer could be immobilized onto a streptavidin surface via its biotin end group, as shown in surface plasmon resonance (SPR) experiments. At 50 °C, at which the block copolymer formed micelles trapping the biotin target within the PNIPMAM core, no immobilization was observed, showing that the biological binding ability of the model could be controlled via external stimuli.

Item Type: Article
Subjects : subj_Chemistry
Authors :
AuthorsEmailORCID
Jochum, FDUNSPECIFIEDUNSPECIFIED
Roth, PJUNSPECIFIEDUNSPECIFIED
Kessler, DUNSPECIFIEDUNSPECIFIED
Theato, PUNSPECIFIEDUNSPECIFIED
Date : 1 September 2010
Identification Number : 10.1021/bm1006085
Copyright Disclaimer : Copyright 2010 American Chemical Society
Uncontrolled Keywords : Science & Technology, Life Sciences & Biomedicine, Physical Sciences, Biochemistry & Molecular Biology, Chemistry, Organic, Polymer Science, Chemistry, BIOCHEMISTRY & MOLECULAR BIOLOGY, CHEMISTRY, ORGANIC, POLYMER SCIENCE, SCHIZOPHRENIC DIBLOCK COPOLYMER, OLIGO(ETHYLENE GLYCOL) METHACRYLATE, TRANSFER RADICAL POLYMERIZATION, ACTIVE ESTER MONOMERS, CORE-SHELL MICROGELS, WATER-SOLUBLE BLOCK, N-ISOPROPYLACRYLAMIDE, AQUEOUS-SOLUTIONS, AGGREGATION BEHAVIOR, RAFT POLYMERIZATION
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 25 May 2016 14:20
Last Modified : 25 May 2016 14:20
URI: http://epubs.surrey.ac.uk/id/eprint/810831

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