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Label-free proteomic analysis of the hydrophobic membrane protein complement in articular chondrocytes: a technique for identification of membrane biomarkers

Matta, C, Zhang, X, Liddell, S, Smith, JR and Mobasheri, A (2015) Label-free proteomic analysis of the hydrophobic membrane protein complement in articular chondrocytes: a technique for identification of membrane biomarkers BIOMARKERS, 20 (8). pp. 572-589.

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Abstract

Context: There is insufficient knowledge about the chondrocyte membranome and its molecular composition. Objective: To develop a Triton X-114 based separation technique using nanoLC-MS/MS combined with shotgun proteomics to identify chondrocyte membrane proteins. Materials and methods: Articular chondrocytes from equine metacarpophalangeal joints were separated into hydrophobic and hydrophilic fractions; trypsin-digested proteins were analysed by nanoLC-MS/MS. Results: A total of 315 proteins were identified. The phase extraction method yielded a high proportion of membrane proteins (56%) including CD276, S100-A6 and three VDAC isoforms. Discussion: Defining the chondrocyte membranome is likely to reveal new biomarker targets for conventional and biological drug discovery.

Item Type: Article
Subjects : Veterinary Medicine
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
AuthorsEmailORCID
Matta, CUNSPECIFIEDUNSPECIFIED
Zhang, XUNSPECIFIEDUNSPECIFIED
Liddell, SUNSPECIFIEDUNSPECIFIED
Smith, JRUNSPECIFIEDUNSPECIFIED
Mobasheri, AUNSPECIFIEDUNSPECIFIED
Date : 17 November 2015
Identification Number : 10.3109/1354750X.2015.1130191
Copyright Disclaimer : This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http:// creativecommons.org/Licenses/by-nc-nd/4.0/), which permits non-com-mercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.
Uncontrolled Keywords : Science & Technology, Life Sciences & Biomedicine, Biotechnology & Applied Microbiology, Toxicology, proteomics, chondrocyte, mass spectrometry, membranome, membrane protein extraction, equine, Triton X-114, Articular cartilage, DIFFERENTIATION IN-VITRO, MESENCHYMAL STEM-CELLS, MASS-SPECTROMETRY, PLASMA-MEMBRANE, CARTILAGE, OSTEOARTHRITIS, EXPRESSION, CHANNELS, MODEL, RECEPTORS
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 06 May 2016 17:43
Last Modified : 06 May 2016 17:43
URI: http://epubs.surrey.ac.uk/id/eprint/810651

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