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Thymic depletion of lymphocytes is associated with the virulence of PRRSV-1 strains

Amarilla, SP, Gomez-Laguna, J, Carrasco, L, Rodriguez-Gomez, IM, Ocerin, JMC, Graham, SP, Frossard, JP, Steinbach, F and Salguero Bodes, FJ (2016) Thymic depletion of lymphocytes is associated with the virulence of PRRSV-1 strains Veterinary Microbiology, 188. pp. 47-58.

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Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) exists as two distinct viruses, type 1 (PRRSV-1) and type 2 (PRRSV-2). Atrophy of the thymus in PRRSV-2 infected piglets has been associated with a loss of thymocytes. The present study aimed to evaluate the impact of PRRSV-1 strains of differing virulence on the thymus of infected piglets by analysing the histomorphometry, the presence of apoptotic cells and cells producing cytokines. Thymic samples were taken from animals experimentally infected (with LV, SU1-bel, and 215-06 strains) or mock inoculated animals at 3, 7 and 35 days post-infection (dpi) and processed for histopathological and immunohistochemical analyses. PRRSV antigen was detected in the thymus from 3dpi until the end of the study in all virus-infected animals with the highest numbers of infected cells detected in SU1-bel group. The histomorphometry analysis and counts of CD3+ thymocytes in the thymic cortex displayed significant differences between strains at different time-points (p ≤ 0.011), with SU1-bel group showing the most severe changes at 7dpi. Cell death displayed statistically significant increase in the cortex of all infected animals, with SU1-bel group showing the highest rate at 3 and 7dpi. The number of cells immunostained against IL-1α, TNF-α and IL-10 were predominantly detected in the medulla (p ≤ 0.01). An increase in the number of TNF-α and IL-10 positive cells was observed in LV and SU-1bel groups. Our results demonstrate that different PRRSV-1 strains induced depletion of the thymic cortex due to apoptosis of thymocytes and that the most severe depletion was associated with the highly virulent SU1-bel strain.

Item Type: Article
Subjects : subj_Veterinary_Medicine
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
AuthorsEmailORCID
Amarilla, SPUNSPECIFIEDUNSPECIFIED
Gomez-Laguna, JUNSPECIFIEDUNSPECIFIED
Carrasco, LUNSPECIFIEDUNSPECIFIED
Rodriguez-Gomez, IMUNSPECIFIEDUNSPECIFIED
Ocerin, JMCUNSPECIFIEDUNSPECIFIED
Graham, SPUNSPECIFIEDUNSPECIFIED
Frossard, JPUNSPECIFIEDUNSPECIFIED
Steinbach, FUNSPECIFIEDUNSPECIFIED
Salguero Bodes, FJUNSPECIFIEDUNSPECIFIED
Date : 2016
Identification Number : 10.1016/j.vetmic.2016.04.005
Copyright Disclaimer : © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Depositing User : Symplectic Elements
Date Deposited : 15 Apr 2016 14:24
Last Modified : 11 May 2016 11:43
URI: http://epubs.surrey.ac.uk/id/eprint/810450

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