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Dissecting Daily and Circadian Expression Rhythms of Clock-Controlled Genes in Human Blood

Lech, K, Ackermann, K, Revell, VL, Lao, O, Skene, DJ and Kayser, M (2016) Dissecting Daily and Circadian Expression Rhythms of Clock-Controlled Genes in Human Blood JOURNAL OF BIOLOGICAL RHYTHMS, 31 (1). pp. 68-81.

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Abstract

The identification and investigation of novel clock-controlled genes (CCGs) has been conducted thus far mainly in model organisms such as nocturnal rodents, with limited information in humans. Here, we aimed to characterize daily and circadian expression rhythms of CCGs in human peripheral blood during a sleep/sleep deprivation (S/SD) study and a constant routine (CR) study. Blood expression levels of 9 candidate CCGs (SREBF1, TRIB1, USF1, THRA1, SIRT1, STAT3, CAPRIN1, MKNK2, and ROCK2), were measured across 48 h in 12 participants in the S/SD study and across 33 h in 12 participants in the CR study. Statistically significant rhythms in expression were observed for STAT3, SREBF1, TRIB1, and THRA1 in samples from both the S/SD and the CR studies, indicating that their rhythmicity is driven by the endogenous clock. The MKNK2 gene was significantly rhythmic in the S/SD but not the CR study, which implies its exogenously driven rhythmic expression. In addition, we confirmed the circadian expression of PER1, PER3, and REV-ERBα in the CR study samples, while BMAL1 and HSPA1B were not significantly rhythmic in the CR samples; all 5 genes previously showed significant expression in the S/SD study samples. Overall, our results demonstrate that rhythmic expression patterns of clock and selected clock-controlled genes in human blood cells are in part determined by exogenous factors (sleep and fasting state) and in part by the endogenous circadian timing system. Knowledge of the exogenous and endogenous regulation of gene expression rhythms is needed prior to the selection of potential candidate marker genes for future applications in medical and forensic settings.

Item Type: Article
Subjects : Biosciences
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine > Department of Biochemical Sciences
Authors :
AuthorsEmailORCID
Lech, KUNSPECIFIEDUNSPECIFIED
Ackermann, KUNSPECIFIEDUNSPECIFIED
Revell, VLUNSPECIFIEDUNSPECIFIED
Lao, OUNSPECIFIEDUNSPECIFIED
Skene, DJUNSPECIFIEDUNSPECIFIED
Kayser, MUNSPECIFIEDUNSPECIFIED
Date : 1 February 2016
Identification Number : 10.1177/0748730415611761
Copyright Disclaimer : Copyright 2016 Sage Publications
Uncontrolled Keywords : Science & Technology, Life Sciences & Biomedicine, Biology, Physiology, Life Sciences & Biomedicine - Other Topics, sleep deprivation, constant routine, gene expression, human blood, clock-controlled genes, HUMAN PERIPHERAL-BLOOD, MONONUCLEAR-CELLS, SLEEP-DEPRIVATION, METABOLIC SYNDROME, LIPID-METABOLISM, THYROID-HORMONE, MESSENGER-RNA, FOOD-INTAKE, T-CELLS, PHASE
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 21 Apr 2016 12:19
Last Modified : 21 Apr 2016 12:19
URI: http://epubs.surrey.ac.uk/id/eprint/810291

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