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Characterization of ascaris from Ecuador and Zanzibar.

Sparks, AM, Betson, M, Oviedo, G, Sandoval, C, Cooper, PJ and Stothard, JR (2015) Characterization of ascaris from Ecuador and Zanzibar. J Helminthol, 89 (4). pp. 512-515.

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Abstract

To shed light on the epidemiology of ascariasis in Ecuador and Zanzibar, 177 adult worms retrieved by chemo-expulsion from either people or pigs were collected, measured and subjected to polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the ribosomal internal transcribed spacer (ITS) region. Upon double digestion with RsaI and HaeIII, PCR-RFLP analysis revealed the presence of A. lumbricoides in people and A. suum in pigs in Ecuador. In contrast, while there are no pigs on Zanzibar, of the 56 worms obtained from people, one was genotyped as A. suum. No additional genetic variation was detected upon further PCR-RFLP analysis with several other restriction enzymes. Upon measurement, worm mass and length differed by location and by species, A. suum being lighter and longer. While there is no evidence to suggest zoonotic transmission in Ecuador, an enduring historical signature of previous zoonotic transmission remains on Zanzibar.

Item Type: Article
Subjects : Veterinary Medicine
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
AuthorsEmailORCID
Sparks, AMUNSPECIFIEDUNSPECIFIED
Betson, MUNSPECIFIEDUNSPECIFIED
Oviedo, GUNSPECIFIEDUNSPECIFIED
Sandoval, CUNSPECIFIEDUNSPECIFIED
Cooper, PJUNSPECIFIEDUNSPECIFIED
Stothard, JRUNSPECIFIEDUNSPECIFIED
Date : July 2015
Identification Number : 10.1017/S0022149X14000431
Copyright Disclaimer : Copyright Cambridge University Press 2014. The online version of this article is published within an Open Access environment subject to the conditions of the Creative Commons Attribution licence http://creativecommons.org/licenses/by/3.0/
Related URLs :
Additional Information : Copyright Cambridge University Press 2014. The online version of this article is published within an Open Access environment subject to the conditions of the Creative Commons Attribution licence http://creativecommons.org/licenses/by/3.0/
Depositing User : Symplectic Elements
Date Deposited : 18 Mar 2016 18:26
Last Modified : 24 Mar 2016 09:13
URI: http://epubs.surrey.ac.uk/id/eprint/810234

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