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Analysis of the transcriptome of the protozoan Theileria parva using MPSS reveals that the majority of genes are transcriptionally active in the schizont stage.

Bishop, R, Shah, T, Pelle, R, Hoyle, D, Pearson, T, Haines, L, Brass, A, Hulme, H, Graham, SP, Taracha, EL, Kanga, S, Lu, C, Hass, B, Wortman, J, White, O, Gardner, MJ, Nene, V and de Villiers, EP (2005) Analysis of the transcriptome of the protozoan Theileria parva using MPSS reveals that the majority of genes are transcriptionally active in the schizont stage. Nucleic Acids Res, 33 (17). pp. 5503-5511.

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Abstract

Massively parallel signature sequencing (MPSS) was used to analyze the transcriptome of the intracellular protozoan Theileria parva. In total 1,095,000, 20 bp sequences representing 4371 different signatures were generated from T.parva schizonts. Reproducible signatures were identified within 73% of potentially detectable predicted genes and 83% had signatures in at least one MPSS cycle. A predicted leader peptide was detected on 405 expressed genes. The quantitative range of signatures was 4-52,256 transcripts per million (t.p.m.). Rare transcripts (<50 t.p.m.) were detected from 36% of genes. Sequence signatures approximated a lognormal distribution, as in microarray. Transcripts were widely distributed throughout the genome, although only 47% of 138 telomere-associated open reading frames exhibited signatures. Antisense signatures comprised 13.8% of the total, comparable with Plasmodium. Eighty five predicted genes with antisense signatures lacked a sense signature. Antisense transcripts were independently amplified from schizont cDNA and verified by sequencing. The MPSS transcripts per million for seven genes encoding schizont antigens recognized by bovine CD8 T cells varied 1000-fold. There was concordance between transcription and protein expression for heat shock proteins that were very highly expressed according to MPSS and proteomics. The data suggests a low level of baseline transcription from the majority of protein-coding genes.

Item Type: Article
Subjects : Veterinary Medicine
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
AuthorsEmailORCID
Bishop, RUNSPECIFIEDUNSPECIFIED
Shah, TUNSPECIFIEDUNSPECIFIED
Pelle, RUNSPECIFIEDUNSPECIFIED
Hoyle, DUNSPECIFIEDUNSPECIFIED
Pearson, TUNSPECIFIEDUNSPECIFIED
Haines, LUNSPECIFIEDUNSPECIFIED
Brass, AUNSPECIFIEDUNSPECIFIED
Hulme, HUNSPECIFIEDUNSPECIFIED
Graham, SPUNSPECIFIEDUNSPECIFIED
Taracha, ELUNSPECIFIEDUNSPECIFIED
Kanga, SUNSPECIFIEDUNSPECIFIED
Lu, CUNSPECIFIEDUNSPECIFIED
Hass, BUNSPECIFIEDUNSPECIFIED
Wortman, JUNSPECIFIEDUNSPECIFIED
White, OUNSPECIFIEDUNSPECIFIED
Gardner, MJUNSPECIFIEDUNSPECIFIED
Nene, VUNSPECIFIEDUNSPECIFIED
de Villiers, EPUNSPECIFIEDUNSPECIFIED
Date : 2005
Identification Number : 10.1093/nar/gki818
Copyright Disclaimer : The Author 2005. Published by Oxford University Press. All rights reserved. The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org
Uncontrolled Keywords : Animals, Genome, Protozoan, Genomics, Open Reading Frames, Protozoan Proteins, RNA, Antisense, RNA, Protozoan, Sequence Analysis, RNA, Telomere, Theileria parva, Transcriptional Activation
Related URLs :
Additional Information : The Author 2005. Published by Oxford University Press. All rights reserved. The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org
Depositing User : Symplectic Elements
Date Deposited : 22 Mar 2016 15:47
Last Modified : 24 Mar 2016 09:36
URI: http://epubs.surrey.ac.uk/id/eprint/810151

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