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Na,K-ATPase Isozymes in Colorectal Cancer and Liver Metastases

Baker Bechmann, M, Rotoli, D, Morales, M, del Carmen Maeso, M, del Pino Garcia, M, Avila, J, Mobasheri, A and Martin-Vasallo, P (2016) Na,K-ATPase Isozymes in Colorectal Cancer and Liver Metastases FRONTIERS IN PHYSIOLOGY, 7, ARTN 9.

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Abstract

The goal of this study was to define Na,K-ATPase α and β subunit isoform expression and isozyme composition in colorectal cancer cells and liver metastases. The α1, α3, and β1 isoforms were the most highly expressed in tumor cells and metastases; in the plasma membrane of non-neoplastic cells and mainly in a cytoplasmic location in tumor cells. α1β1 and α3β1 isozymes found in tumor and metastatic cells exhibit the highest and lowest Na(+) affinity respectively and the highest K(+) affinity. Mesenchymal cell isozymes possess an intermediate Na(+) affinity and a low K(+) affinity. In cancer, these ions are likely to favor optimal conditions for the function of nuclear enzymes involved in mitosis, especially a high intra-nuclear K(+) concentration. A major and striking finding of this study was that in liver, metastasized CRC cells express the α3β1 isozyme. Thus, the α3β1 isozyme could potentially serve as a novel exploratory biomarker of CRC metastatic cells in liver.

Item Type: Article
Subjects : Biochemistry; Physiology
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
AuthorsEmailORCID
Baker Bechmann, MUNSPECIFIEDUNSPECIFIED
Rotoli, DUNSPECIFIEDUNSPECIFIED
Morales, MUNSPECIFIEDUNSPECIFIED
del Carmen Maeso, MUNSPECIFIEDUNSPECIFIED
del Pino Garcia, MUNSPECIFIEDUNSPECIFIED
Avila, JUNSPECIFIEDUNSPECIFIED
Mobasheri, AUNSPECIFIEDUNSPECIFIED
Martin-Vasallo, PUNSPECIFIEDUNSPECIFIED
Date : 29 January 2016
Identification Number : 10.3389/fphys.2016.00009
Uncontrolled Keywords : Science & Technology, Life Sciences & Biomedicine, Physiology, Na/K-ATPase isozymes, sodium pump isozymes, colorectal cancer, colorectal cancer liver metastases, Na/K-ATPase isoforms colorectal cancer immunohistochemistry, NA+-K+-ATPASE, TISSUE-SPECIFIC EXPRESSION, CENTRAL-NERVOUS-SYSTEM, BETA 3 SUBUNIT, CELLULAR-LOCALIZATION, CALCIUM OSCILLATIONS, ADHESION MOLECULE, SKELETAL-MUSCLE, ALPHA-SUBUNIT, ISOFORMS
Related URLs :
Additional Information : Copyright © 2016 Baker Bechmann, Rotoli, Morales, Maeso, García, Ávila, Mobasheri and Martín-Vasallo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Depositing User : Symplectic Elements
Date Deposited : 09 Mar 2016 10:27
Last Modified : 09 Mar 2016 10:27
URI: http://epubs.surrey.ac.uk/id/eprint/810063

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