University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Characterization of human skeletal stem and bone cell populations using dielectrophoresis

Ismail, A, Hughes, M, Mulhall, H, Oreffo, R and Labeed, F (2015) Characterization of human skeletal stem and bone cell populations using dielectrophoresis Journal of Tissue Engineering and Regenerative Medicine, 9 (2). pp. 162-168.

[img] Text (deleted)
Ismail et al 2016.doc - Author's Original
Restricted to Repository staff only
Available under License : See the attached licence file.

Download (1MB)
[img] Text
Ismail et al 2015.doc - Author's Original
Restricted to Repository staff only
Available under License : See the attached licence file.

Download (1MB)
[img]
Preview
Text (licence)
SRI_deposit_agreement.pdf
Available under License : See the attached licence file.

Download (33kB) | Preview
[img]
Preview
Text
Ismail et al 2015.pdf

Download (538kB) | Preview

Abstract

© 2012 John Wiley & Sons, Ltd. Dielectrophoresis (DEP) is a non-invasive cell analysis method that uses differences in electrical properties between particles and surrounding medium to determine a unique set of cellular properties that can be used as a basis for cell separation. Cell-based therapies using skeletal stem cells are currently one of the most promising areas for treating a variety of skeletal and muscular disorders. However, identifying and sorting these cells remains a challenge in the absence of unique skeletal stem cell markers. DEP provides an ideal method for identifying subsets of cells without the need for markers by using their dielectric properties. This study used a 3D dielectrophoretic well chip device to determine the dielectric characteristics of two osteosarcoma cell lines (MG-63 and SAOS-2) and an immunoselected enriched skeletal stem cell fraction (STRO-1 positive cell) of human bone marrow. Skeletal cells were exposed to a series of different frequencies to induce dielectrophoretic cell movement, and a model was developed to generate the membrane and cytoplasmic properties of the cell populations. Differences were observed in the dielectric properties of MG-63, SAOS-2 and STRO-1 enriched skeletal populations, which could potentially be used to sort cells in mixed populations. This study provide evidence of the ability to characterize different human skeletal stem and mature cell populations, and acts as a proof-of-concept that dielectrophoresis can be exploited to detect, isolate and separate skeletal cell populations from heterogeneous bone marrow cell populations.

Item Type: Article
Subjects : Biomedical engineering
Divisions : Faculty of Engineering and Physical Sciences > Mechanical Engineering Sciences
Authors :
AuthorsEmailORCID
Ismail, AUNSPECIFIEDUNSPECIFIED
Hughes, MUNSPECIFIEDUNSPECIFIED
Mulhall, HUNSPECIFIEDUNSPECIFIED
Oreffo, RUNSPECIFIEDUNSPECIFIED
Labeed, FUNSPECIFIEDUNSPECIFIED
Date : 1 January 2015
Identification Number : 10.1002/term.1629
Copyright Disclaimer : This is the peer reviewed version of the following article: Ismail, A, Hughes, M, Mulhall, H, Oreffo, R and Labeed, F (2015) Characterization of human skeletal stem and bone cell populations using dielectrophoresis Journal of Tissue Engineering and Regenerative Medicine, 9 (2). 162 - 168, which has been published in final form at http://dx.doi.org/10.1002/term.1629. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Additional Information : This is the peer reviewed version of the following article: Ismail, A, Hughes, M, Mulhall, H, Oreffo, R and Labeed, F (2015) Characterization of human skeletal stem and bone cell populations using dielectrophoresis Journal of Tissue Engineering and Regenerative Medicine, 9 (2). 162 - 168, which has been published in final form at http://dx.doi.org/10.1002/term.1629. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Depositing User : Symplectic Elements
Date Deposited : 01 Mar 2016 17:20
Last Modified : 01 Mar 2016 17:24
URI: http://epubs.surrey.ac.uk/id/eprint/809993

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800