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Genotyping of Listeria monocytogenes isolates from poultry carcasses using high resolution melting (HRM) analysis.

Sakaridis, I, Ganopoulos, I, Madesis, P, Tsaftaris, A and Argiriou, A (2014) Genotyping of Listeria monocytogenes isolates from poultry carcasses using high resolution melting (HRM) analysis. Biotechnology & Biotechnological Equipment, 28 (1). pp. 107-111.

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Abstract

An outbreak situation of human listeriosis requires a fast and accurate protocol for typing Listeria monocytogenes. Existing techniques are either characterized by low discriminatory power or are laborious and require several days to give a final result. Polymerase chain reaction (PCR) coupled with high resolution melting (HRM) analysis was investigated in this study as an alternative tool for a rapid and precise genotyping of L. monocytogenes isolates. Fifty-five isolates of L. monocytogenes isolated from poultry carcasses and the environment of four slaughterhouses were typed by HRM analysis using two specific markers, internalin B and ssrA genes. The analysis of genotype confidence percentage of L. monocytogenes isolates produced by HRM analysis generated dendrograms with two major groups and several subgroups. Furthermore, the analysis of the HRM curves revealed that all L. monocytogenes isolates could easily be distinguished. In conclusion, HRM was proven to be a fast and powerful tool for genotyping isolates of L. monocytogenes.

Item Type: Article
Subjects : Veterinary public health
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
AuthorsEmailORCID
Sakaridis, IUNSPECIFIEDUNSPECIFIED
Ganopoulos, IUNSPECIFIEDUNSPECIFIED
Madesis, PUNSPECIFIEDUNSPECIFIED
Tsaftaris, AUNSPECIFIEDUNSPECIFIED
Argiriou, AUNSPECIFIEDUNSPECIFIED
Date : 6 May 2014
Identification Number : 10.1080/13102818.2014.901681
Copyright Disclaimer : Copyright 2014 The Author(s). Published by Taylor & Francis. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.
Uncontrolled Keywords : HRM, L. monocytogenes, RAPD, genotyping, poultry carcasses
Related URLs :
Additional Information : Copyright 2014 The Author(s). Published by Taylor & Francis. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.
Depositing User : Symplectic Elements
Date Deposited : 23 Feb 2016 14:31
Last Modified : 23 Feb 2016 15:46
URI: http://epubs.surrey.ac.uk/id/eprint/809846

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