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High resolution melting analysis for quantitative detection of bovine milk in pure water buffalo mozzarella and other buffalo dairy products

Sakaridis, I, Ganopoulos, I, Argiriou, A and Tsaftaris, A (2013) High resolution melting analysis for quantitative detection of bovine milk in pure water buffalo mozzarella and other buffalo dairy products International Dairy Journal.

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Abstract

Identification of buffalo dairy products has become an important issue to ascertain product quality, consumer rights and absence of food-borne allergic reactions. A polymerase chain reaction (PCR) followed by a high resolution melting (HRM) analysis was developed and applied for species specific detection of bovine milk in nine different commercial buffalo dairy products. A specific buffalo 12S rRNA and a bovine d-loop primer pair, targeting the mitochondrial genome, were employed in a duplex PCR assay. The analysis developed was found capable of identifying the presence of bovine milk down to 1% in commercial buffalo milk products and also of quantifying the ratio of bovine into buffalo milk. HRM was proven to be a fast and accurate technique for a routine authentication testing of mozzarella and other buffalo milk products.

Item Type: Article
Authors :
NameEmailORCID
Sakaridis, IUNSPECIFIEDUNSPECIFIED
Ganopoulos, IUNSPECIFIEDUNSPECIFIED
Argiriou, AUNSPECIFIEDUNSPECIFIED
Tsaftaris, AUNSPECIFIEDUNSPECIFIED
Date : 8 January 2013
Identification Number : 10.1016/j.idairyj.2012.08.006
Depositing User : Symplectic Elements
Date Deposited : 28 Mar 2017 10:57
Last Modified : 31 Oct 2017 18:01
URI: http://epubs.surrey.ac.uk/id/eprint/809843

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