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Protection Induced by Simultaneous Subcutaneous and Endobronchial Vaccination with BCG/BCG and BCG/Adenovirus Expressing Antigen 85A against Mycobacterium bovis in Cattle

Dean, GS, Clifford, D, Whelan, AO, Tchilian, EZ, Beverley, PCL, Salguero, FJ, Xing, Z, Vordermeier, HM and Villarreal-Ramos, B (2015) Protection Induced by Simultaneous Subcutaneous and Endobronchial Vaccination with BCG/BCG and BCG/Adenovirus Expressing Antigen 85A against Mycobacterium bovis in Cattle PLoS One, 10 (11), ARTN e.

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Abstract

The incidence of bovine tuberculosis (bTB) in the GB has been increasing since the 1980s. Immunisation, alongside current control measures, has been proposed as a sustainable measure to control bTB. Immunisation with Mycobacterium bovis bacillus Calmette-Guerin (BCG) has been shown to protect against bTB. Furthermore, much experimental data indicates that pulmonary local immunity is important for protection against respiratory infections including Mycobacterium tuberculosis and that pulmonary immunisation is highly effective. Here, we evaluated protection against M. bovis, the main causative agent of bTB, conferred by BCG delivered subcutaneously, endobronchially or by the new strategy of simultaneous immunisation by both routes. We also tested simultaneous subcutaneous immunisation with BCG and endobronchial delivery of a recombinant type 5 adenovirus expressing mycobacterial antigen 85A. There was significantly reduced visible pathology in animals receiving the simultaneous BCG/BCG or BCG/Ad85 treatment compared to naïve controls. Furthermore, there were significantly fewer advanced microscopic granulomata in animals receiving BCG/Ad85A compared to naive controls. Thus, combining local and systemic immunisation limits the development of pathology, which in turn could decrease bTB transmission.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
AuthorsEmailORCID
Dean, GSUNSPECIFIEDUNSPECIFIED
Clifford, DUNSPECIFIEDUNSPECIFIED
Whelan, AOUNSPECIFIEDUNSPECIFIED
Tchilian, EZUNSPECIFIEDUNSPECIFIED
Beverley, PCLUNSPECIFIEDUNSPECIFIED
Salguero, FJUNSPECIFIEDUNSPECIFIED
Xing, ZUNSPECIFIEDUNSPECIFIED
Vordermeier, HMUNSPECIFIEDUNSPECIFIED
Villarreal-Ramos, BUNSPECIFIEDUNSPECIFIED
Date : 6 November 2015
Identification Number : 10.1371/journal.pone.0142270
Uncontrolled Keywords : Science & Technology, Multidisciplinary Sciences, Science & Technology - Other Topics, PULMONARY TUBERCULOSIS, BCG, IMMUNIZATION, RESPONSES, VACCINES, MUCOSAL, CELLS, MICE
Related URLs :
Additional Information : Copyright: © 2015 Dean et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Depositing User : Symplectic Elements
Date Deposited : 08 Jan 2016 11:36
Last Modified : 08 Jan 2016 11:36
URI: http://epubs.surrey.ac.uk/id/eprint/809660

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