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Conserved mRNA-binding proteomes in eukaryotic organisms

Matia-González, AM, Laing, EE and Gerber, AP (2015) Conserved mRNA-binding proteomes in eukaryotic organisms Nature Structural and Molecular Biology, 22 (12). pp. 1027-1033.

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Abstract

RNA-binding proteins (RBPs) are essential for post-transcriptional regulation of gene expression. Recent high-throughput screens have dramatically increased the number of experimentally identified RBPs; however, comprehensive identification of RBPs within living organisms is elusive. Here we describe the repertoire of 765 and 594 proteins that reproducibly interact with polyadenylated mRNAs in Saccharomyces cerevisiae and Caenorhabditis elegans, respectively. Furthermore, we report the differential association of mRNA-binding proteins (mRPBs) upon induction of apoptosis in C. elegans L4-stage larvae. Strikingly, most proteins composing mRBPomes, including components of early metabolic pathways and the proteasome, are evolutionarily conserved between yeast and C. elegans. We speculate, on the basis of our evidence that glycolytic enzymes bind distinct glycolytic mRNAs, that enzyme-mRNA interactions relate to an ancient mechanism for post-transcriptional coordination of metabolic pathways that perhaps was established during the transition from the early 'RNA world' to the 'protein world'.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine > Department of Microbial and Cellular Sciences
Authors :
AuthorsEmailORCID
Matia-González, AMUNSPECIFIEDUNSPECIFIED
Laing, EEUNSPECIFIEDUNSPECIFIED
Gerber, APUNSPECIFIEDUNSPECIFIED
Date : 23 November 2015
Identification Number : 10.1038/nsmb.3128
Additional Information : Copyright 2015 Nature America Inc. All rights reserved.
Depositing User : Symplectic Elements
Date Deposited : 23 Dec 2015 09:39
Last Modified : 23 May 2016 01:08
URI: http://epubs.surrey.ac.uk/id/eprint/809539

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