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X-ray micro-computed tomography in willow reveals tissue patterning of reaction wood and delay in programmed cell death

Murphy, RJ (2015) X-ray micro-computed tomography in willow reveals tissue patterning of reaction wood and delay in programmed cell death BMC Plant Biology.

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Brereton et al X-ray tomography willows reaction wood BMC Plant Biology 2015.pdf - ["content_typename_Published version (Publisher's proof or final PDF)" not defined]
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Abstract

Abstract Background: Variation in the reaction wood (RW) response has been shown to be a principle component driving differences in lignocellulosic sugar yield from the bioenergy crop willow. The phenotypic cause(s) behind these differences in sugar yield, beyond their common elicitor, however, remain unclear. Here we use X-ray micro-computed tomography (μCT) to investigate RW-associated alterations in secondary xylem tissue patterning in three dimensions (3D). Results: Major architectural alterations were successfully quantified in 3D and attributed to RW induction. Whilst the frequency of vessels was reduced in tension wood tissue (TW), the total vessel volume was significantly increased. Interestingly, a delay in programmed-cell-death (PCD) associated with TW was also clearly observed and readily quantified by μCT. Conclusions: The surprising degree to which the volume of vessels was increased illustrates the substantial xylem tissue remodelling involved in reaction wood formation. The remodelling suggests an important physiological compromise between structural and hydraulic architecture necessary for extensive alteration of biomass and helps to demonstrate the power of improving our perspective of cell and tissue architecture. The precise observation of xylem tissue development and quantification

Item Type: Article
Divisions : Faculty of Engineering and Physical Sciences > Centre for Environmental Strategy
Authors :
AuthorsEmailORCID
Murphy, RJUNSPECIFIEDUNSPECIFIED
Date : 11 March 2015
Identification Number : 10.1186/s12870-015-0438-0
Additional Information : © 2015 The authors. licensee BioMed Central. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Depositing User : Symplectic Elements
Date Deposited : 12 Jan 2016 16:16
Last Modified : 12 Jan 2016 16:16
URI: http://epubs.surrey.ac.uk/id/eprint/809513

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