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Assessing the suitability of miRNA-142-5p and miRNA-541 for bloodstain deposition timing

Lech, K, Ackermann, K, Wollstein, A, Revell, VL, Skene, DJ and Kayser, M (2014) Assessing the suitability of miRNA-142-5p and miRNA-541 for bloodstain deposition timing FORENSIC SCIENCE INTERNATIONAL-GENETICS, 12. pp. 181-184.

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Abstract

A recent proof-of-concept pilot study proposed using microRNA (miRNA) markers for time of death determination. The markers – miRNA-142-5p and miRNA-541, were reported to show considerable expression differences in vitreous humor between individuals who died during the day or night. Here, we investigated whether these miRNA markers show the same diurnal expression pattern in blood, which would make them useful for estimating bloodstain deposition time to allow molecular alibi testing for forensic casework. We analyzed venous blood samples collected from 12 healthy individuals every 4 h during the 24 h day/night period under controlled sleep-laboratory conditions. MiRNA-142-5p normalized against miRNA-222 showed no statistically significant expression differences between blood samples collected during daytime and nighttime (one-way ANOVA p = 0.81), and also no statistically significant rhythmicity during the 24 h day/night period (cosine fit for all individuals p > 0.05, averaged data p = 0.932). MicroRNA-541 amplification in blood was above the 34-cycle threshold applied in the study, indicating too low quantities for obtaining reliable data. Overall, we conclude that the two miRNA markers previously suggested for time of death determination in vitreous humor are not suitable for estimating the deposition time of forensic bloodstains. Future studies may find out if miRNA markers with significant diurnal expression patterns can be identified and how useful they would be for forensic trace deposition timing.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine > Department of Biochemical Sciences
Authors :
AuthorsEmailORCID
Lech, KUNSPECIFIEDUNSPECIFIED
Ackermann, KUNSPECIFIEDUNSPECIFIED
Wollstein, AUNSPECIFIEDUNSPECIFIED
Revell, VLUNSPECIFIEDUNSPECIFIED
Skene, DJUNSPECIFIEDUNSPECIFIED
Kayser, MUNSPECIFIEDUNSPECIFIED
Date : 1 September 2014
Identification Number : 10.1016/j.fsigen.2014.06.008
Uncontrolled Keywords : Science & Technology, Life Sciences & Biomedicine, Genetics & Heredity, Medicine, Legal, Legal Medicine, MicroRNA, Circadian, Biomarkers, Trace timing, CIRCADIAN-CLOCK, SLEEP-DEPRIVATION, GENE-EXPRESSION, TIME, MICRORNAS, MELATONIN, RHYTHMS, PHYSIOLOGY, GENOMICS, BIOLOGY
Related URLs :
Additional Information : © 2014. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Depositing User : Symplectic Elements
Date Deposited : 08 Dec 2015 12:15
Last Modified : 08 Dec 2015 12:15
URI: http://epubs.surrey.ac.uk/id/eprint/809426

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