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Genome-Wide Analysis of In Vivo Binding of the Master Regulator DasR in Streptomyces coelicolor Identifies Novel Non-Canonical Targets

Swiatek-Polatynska, MA, Bucca, G, Laing, E, Gubbens, J, Titgemeyer, F, Smith, CP, Rigali, S and van Wezel, GP (2015) Genome-Wide Analysis of In Vivo Binding of the Master Regulator DasR in Streptomyces coelicolor Identifies Novel Non-Canonical Targets PLOS ONE, 10 (4), ARTN e.

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Abstract

Streptomycetes produce a wealth of natural products, including over half of all known antibiotics. It was previously demonstrated that N-acetylglucosamine and secondary metabolism are closely entwined in streptomycetes. Here we show that DNA recognition by the N-acetylglucosamine-responsive regulator DasR is growth-phase dependent, and that DasR can bind to sites in the S. coelicolor genome that have no obvious resemblance to previously identified DasR-responsive elements. Thus, the regulon of DasR extends well beyond what was previously predicted and includes a large number of genes with functions far removed from N-acetylglucosamine metabolism, such as genes for small RNAs and DNA transposases. Conversely, the DasR regulon during vegetative growth largely correlates to the presence of canonical DasR-responsive elements. The changes in DasR binding in vivo following N-acetylglucosamine induction were studied in detail and a possible molecular mechanism by which the influence of DasR is extended is discussed. Discussion of DasR binding was further informed by a parallel transcriptome analysis of the respective cultures. Evidence is provided that DasR binds directly to the promoters of all genes encoding pathway-specific regulators of antibiotic production in S. coelicolor, thereby providing an exquisitely simple link between nutritional control and secondary metabolism.

Item Type: Article
Authors :
AuthorsEmailORCID
Swiatek-Polatynska, MAUNSPECIFIEDUNSPECIFIED
Bucca, GUNSPECIFIEDUNSPECIFIED
Laing, EUNSPECIFIEDUNSPECIFIED
Gubbens, JUNSPECIFIEDUNSPECIFIED
Titgemeyer, FUNSPECIFIEDUNSPECIFIED
Smith, CPUNSPECIFIEDUNSPECIFIED
Rigali, SUNSPECIFIEDUNSPECIFIED
van Wezel, GPUNSPECIFIEDUNSPECIFIED
Date : 15 April 2015
Identification Number : 10.1371/journal.pone.0122479
Uncontrolled Keywords : Science & Technology, Multidisciplinary Sciences, Science & Technology - Other Topics, AERIAL MYCELIUM FORMATION, N-ACETYLGLUCOSAMINE METABOLISM, CARBON CATABOLITE REPRESSION, AMP RECEPTOR PROTEIN, ANTIBIOTIC PRODUCTION, ESCHERICHIA-COLI, MORPHOLOGICAL-DIFFERENTIATION, PHOSPHOTRANSFERASE SYSTEM, BACILLUS-SUBTILIS, GLUCOSE KINASE
Related URLs :
Additional Information : Published underthe Creative Commons Attribution (CC BY 4.0) license
Depositing User : Symplectic Elements
Date Deposited : 11 Nov 2015 16:22
Last Modified : 11 Nov 2015 16:22
URI: http://epubs.surrey.ac.uk/id/eprint/809060

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