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Expression of Transient Receptor Potential Vanilloid (TRPV) channels in different passages of articular chondrocytes.

Hdud, IM, El-Shafei, AA, Loughna, P, Barrett-Jolley, R and Mobasheri, A (2012) Expression of Transient Receptor Potential Vanilloid (TRPV) channels in different passages of articular chondrocytes. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 13 (4). pp. 4433-4445.

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Abstract

Ion channels play important roles in chondrocyte mechanotransduction. The transient receptor potential vanilloid (TRPV) subfamily of ion channels consists of six members. TRPV1-4 are temperature sensitive calcium-permeable, relatively non-selective cation channels whereas TRPV5 and TRPV6 show high selectivity for calcium over other cations. In this study we investigated the effect of time in culture and passage number on the expression of TRPV4, TRPV5 and TRPV6 in articular chondrocytes isolated from equine metacarpophalangeal joints. Polyclonal antibodies raised against TRPV4, TRPV5 and TRPV6 were used to compare the expression of these channels in lysates from first expansion chondrocytes (P0) and cells from passages 1-3 (P1, P2 and P3) by western blotting. TRPV4, TRPV5 and TRPV6 were expressed in all passages examined. Immunohistochemistry and immunofluorescence confirmed the presence of these channels in sections of formalin fixed articular cartilage and monolayer cultures of methanol fixed P2 chondrocytes. TRPV5 and TRPV6 were upregulated with time and passage in culture suggesting that a shift in the phenotype of the cells in monolayer culture alters the expression of these channels. In conclusion, several TRPV channels are likely to be involved in calcium signaling and homeostasis in chondrocytes.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
AuthorsEmailORCID
Hdud, IMUNSPECIFIEDUNSPECIFIED
El-Shafei, AAUNSPECIFIEDUNSPECIFIED
Loughna, PUNSPECIFIEDUNSPECIFIED
Barrett-Jolley, RUNSPECIFIEDUNSPECIFIED
Mobasheri, AUNSPECIFIEDUNSPECIFIED
Date : 10 April 2012
Identification Number : 10.3390/ijms13044433
Uncontrolled Keywords : TRPV4, TRPV5, TRPV6, cartilage, chondrocyte, culture, dedifferentiation, immunofluorescence, immunohistochemistry, mechanotransduction, passage, Animals, Calcium, Calcium Signaling, Cartilage, Articular, Cation Transport Proteins, Cell Dedifferentiation, Cell Membrane, Cells, Cultured, Chondrocytes, Gene Expression Regulation, Horses, Mechanotransduction, Cellular, TRPV Cation Channels
Related URLs :
Additional Information : © 2012 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
Depositing User : Symplectic Elements
Date Deposited : 07 Oct 2015 17:23
Last Modified : 07 Oct 2015 17:23
URI: http://epubs.surrey.ac.uk/id/eprint/808698

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