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Ameliorative Effects of PACAP against Cartilage Degeneration. Morphological, Immunohistochemical and Biochemical Evidence from in Vivo and in Vitro Models of Rat Osteoarthritis

Giunta, S, Castorina, A, Marzagalli, R, Szychlinska, MA, Pichler, K, Mobasheri, A and Musumeci, G (2015) Ameliorative Effects of PACAP against Cartilage Degeneration. Morphological, Immunohistochemical and Biochemical Evidence from in Vivo and in Vitro Models of Rat Osteoarthritis INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 16 (3). pp. 5922-5944.

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Ameliorative effects of PACAP against cartilage degeneration. Morphological, immunohistochemical and biochemical evidence from in vivo and in vitro models of rat osteoarthritis..pdf - ["content_typename_Published version (Publisher's proof or final PDF)" not defined]
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Abstract

Osteoarthritis (OA); the most common form of degenerative joint disease, is associated with variations in pro-inflammatory growth factor levels, inflammation and hypocellularity resulting from chondrocyte apoptosis. Pituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide endowed with a range of trophic effects in several cell types; including chondrocytes. However; its role in OA has not been studied. To address this issue, we investigated whether PACAP expression is affected in OA cartilage obtained from experimentally-induced OA rat models, and then studied the effects of PACAP in isolated chondrocytes exposed to IL-1β in vitro to mimic the inflammatory milieu of OA cartilage. OA induction was established by histomorphometric and histochemical analyses. Changes in PACAP distribution in cartilage, or its concentration in synovial fluid (SF), were assessed by immunohistochemistry and ELISA. Results showed that PACAP abundance in cartilage tissue and SF was high in healthy controls. OA induction decreased PACAP levels both in affected cartilage and SF. In vitro, PACAP prevented IL-1β-induced chondrocyte apoptosis, as determined by MTT assay; Hoechst staining and western blots of apoptotic-related proteins. These changes were also accompanied by decreased i-NOS and COX-2 levels, suggesting an anti-inflammatory effect. Altogether, these findings support a potential role for PACAP as a chondroprotective agent for the treatment of OA.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
AuthorsEmailORCID
Giunta, SUNSPECIFIEDUNSPECIFIED
Castorina, AUNSPECIFIEDUNSPECIFIED
Marzagalli, RUNSPECIFIEDUNSPECIFIED
Szychlinska, MAUNSPECIFIEDUNSPECIFIED
Pichler, KUNSPECIFIEDUNSPECIFIED
Mobasheri, AUNSPECIFIEDUNSPECIFIED
Musumeci, GUNSPECIFIEDUNSPECIFIED
Date : 1 March 2015
Identification Number : 10.3390/ijms16035922
Uncontrolled Keywords : Science & Technology, Life Sciences & Biomedicine, Physical Sciences, Biochemistry & Molecular Biology, Chemistry, Multidisciplinary, Chemistry, VASOACTIVE-INTESTINAL-PEPTIDE, CYCLASE-ACTIVATING PEPTIDE, COLLAGEN-INDUCED ARTHRITIS, MESENCHYMAL STEM-CELLS, ARTICULAR-CARTILAGE, SYNOVIAL FIBROBLASTS, LUBRICIN EXPRESSION, DIABETIC-RATS, KNEE MENISCI, NEURODEGENERATIVE DISEASES
Related URLs :
Additional Information : © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
Depositing User : Symplectic Elements
Date Deposited : 07 Oct 2015 08:47
Last Modified : 07 Oct 2015 08:47
URI: http://epubs.surrey.ac.uk/id/eprint/808679

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