Measuring the dielectric properties of herpes simplex virus type 1 virions with dielectrophoresis
Hughes, MP, Morgan, H and Rixon, FJ (2002) Measuring the dielectric properties of herpes simplex virus type 1 virions with dielectrophoresis BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1571 (1), PII S0. pp. 1-8.
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An investigation has been performed into the biophysical properties of the enveloped mammalian virus, herpes simplex virus type 1 (HSV-1). The dielectrophoretic behaviour of the virus particles was measured as a function of applied frequency (over the range 100 kHz-20 MHz) and conductivity of the suspending medium (over the range 1-100 mS m(-1)). The dielectric properties of the virus were determined from the dielectrophoretic data using the smeared-out shell model. The data suggest that the intact particle has a surface conductance of 0.3 nS, an internal and membrane permittivity of 75varepsilon(o) and 7.5varepsilon(o), respectively, an internal conductivity of approximately 0.1 S m(-1) and a zeta potential of 70 mV. The dielectric properties were measured for intact, fresh virus particles and also for particles following exposure to various modifying agents, such as treatment with enzymes, ionophores and ageing. It is shown that the observed changes in the dielectrophoretic spectrum, and the variations in the dielectric properties of the virus concur with the expected physiological effects of these agents.
|Divisions :||Faculty of Engineering and Physical Sciences > Mechanical Engineering Sciences|
|Date :||10 May 2002|
|Identification Number :||10.1016/S0304-4165(02)00161-7|
|Uncontrolled Keywords :||Science & Technology, Life Sciences & Biomedicine, Biochemistry & Molecular Biology, Biophysics, BIOCHEMISTRY & MOLECULAR BIOLOGY, BIOPHYSICS, herpes simplex virus type 1, virion, dielectrophoresis, AC electrokinetics, SUBMICROMETER LATEX SPHERES, MANIPULATION, BIOPARTICLES, ELECTROROTATION, SEPARATION, MEMBRANES, PARTICLES, CELLS|
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|Additional Information :||© 2002. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ Taylor & Francis also have a list of journal-specific embargoes here|
|Depositing User :||Symplectic Elements|
|Date Deposited :||29 Sep 2015 10:41|
|Last Modified :||29 Sep 2015 10:41|
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