University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Dielectrophoretic sample preparation for environmental monitoring of microorganisms: Soil particle removal

Fatoyinbo, HO, McDonnell, MC and Hughes, MP (2014) Dielectrophoretic sample preparation for environmental monitoring of microorganisms: Soil particle removal BIOMICROFLUIDICS, 8 (4), ARTN 0.

[img] Text
Fatoyinbo et al - final version.docx
Restricted to Repository staff only
Available under License : See the attached licence file.

Download (582kB)
[img]
Preview
Text (licence)
SRI_deposit_agreement.pdf
Available under License : See the attached licence file.

Download (33kB) | Preview
[img]
Preview
Text
Dielectrophoretic sample preparation .pdf

Download (917kB) | Preview

Abstract

Detection of pathogens from environmental samples is often hampered by sensors interacting with environmental particles such as soot, pollen, or environmental dust such as soil or clay. These particles may be of similar size to the target bacterium, preventing removal by filtration, but may non-specifically bind to sensor surfaces, fouling them and causing artefactual results. In this paper, we report the selective manipulation of soil particles using an AC electrokinetic microfluidic system. Four heterogeneous soil samples (smectic clay, kaolinitic clay, peaty loam, and sandy loam) were characterised using dielectrophoresis to identify the electrical difference to a target organism. A flow-cell device was then constructed to evaluate dielectrophoretic separation of bacteria and clay in a continous flow through mode. The average separation efficiency of the system across all soil types was found to be 68.7% with a maximal separation efficiency for kaolinitic clay at 87.6%. This represents the first attempt to separate soil particles from bacteria using dielectrophoresis and indicate that the technique shows significant promise; with appropriate system optimisation, we believe that this preliminary study represents an opportunity to develop a simple yet highly effective sample processing system.

Item Type: Article
Divisions : Faculty of Engineering and Physical Sciences > Mechanical Engineering Sciences
Authors :
AuthorsEmailORCID
Fatoyinbo, HOUNSPECIFIEDUNSPECIFIED
McDonnell, MCUNSPECIFIEDUNSPECIFIED
Hughes, MPUNSPECIFIEDUNSPECIFIED
Date : 1 July 2014
Identification Number : 10.1063/1.4892036
Uncontrolled Keywords : Science & Technology, Life Sciences & Biomedicine, Physical Sciences, Biochemical Research Methods, Biophysics, Nanoscience & Nanotechnology, Physics, Fluids & Plasmas, Biochemistry & Molecular Biology, Science & Technology - Other Topics, Physics, DENSITY GRADIENT CENTRIFUGATION, ELECTRICAL-PROPERTIES, SEPARATION, BACTERIA, EXTRACTION, WATER, CONDUCTIVITY, ELUTRIATION, MICROFLORAS, DISPERSION
Related URLs :
Additional Information : Copyright 2014 American Institute of Physics
Depositing User : Symplectic Elements
Date Deposited : 25 Sep 2015 17:40
Last Modified : 25 Sep 2015 17:40
URI: http://epubs.surrey.ac.uk/id/eprint/808501

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800