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Rapid determination of antibiotic resistance in E-coli using dielectrophoresis

Hoettges, KF, Dale, JW and Hughes, MP (2007) Rapid determination of antibiotic resistance in E-coli using dielectrophoresis PHYSICS IN MEDICINE AND BIOLOGY, 52 (19). pp. 6001-6009.

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Abstract

In recent years, infections due to antibiotic-resistant strains of bacteria such as methillicin-resistant Staphylococcus aureus and ciprofloxacin-resistant Escherichia coli are on the rise, and with them the demand for rapid antibiotic testing is also rising. Conventional tests, such as disc diffusion testing, require a primary sample to be tested in the presence of a number of antibiotics to verify which antibiotics suppress growth, which take approximately 24 h to complete and potentially place the patient at severe risk. In this paper we describe the use of dielectrophoresis as a rapid marker of cell death, by detecting changes in the electrophysiology of the cell caused by the administration of an antibiotic. In contrast to other markers, the electrophysiology of the cell changes rapidly during cell death allowing live cells to be distinguished from dead (or dying) cells without the need for culturing. Using polymyxin B as an example antibiotic, our studies indicate that significant changes in cell characteristics can be observed as soon as 1 h passes after isolating a culture from nutrient broth.

Item Type: Article
Divisions : Faculty of Engineering and Physical Sciences > Mechanical Engineering Sciences
Authors :
AuthorsEmailORCID
Hoettges, KFUNSPECIFIEDUNSPECIFIED
Dale, JWUNSPECIFIEDUNSPECIFIED
Hughes, MPUNSPECIFIEDUNSPECIFIED
Date : 7 October 2007
Identification Number : 10.1088/0031-9155/52/19/019
Uncontrolled Keywords : Science & Technology, Technology, Life Sciences & Biomedicine, Engineering, Biomedical, Radiology, Nuclear Medicine & Medical Imaging, Engineering, ENGINEERING, BIOMEDICAL, RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
Related URLs :
Additional Information : This is an author-created, un-copyedited version of an article accepted for publication in Physics in Medicine and Biology. The publisher is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at 10.1088/0031-9155/52/19/019.
Depositing User : Symplectic Elements
Date Deposited : 02 Oct 2015 13:02
Last Modified : 02 Oct 2015 13:02
URI: http://epubs.surrey.ac.uk/id/eprint/808499

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